Sr. Research Associate, Protein Design

RXinsider LTD.; Cambridge (MA); On-site; USD 74,000 - 118,000; Full time

Benefits offered by this job

Competitive healthcare and voluntary福利

Well‑being resources including fitness

Family planning benefits (fertility, -

Sabbatical and global recharge days

Generous paid time off

Job summary

Moderna in Cambridge, MA is seeking a Senior Research Associate for the Protein Design team to advance vaccine and therapeutic programs through protein production, purification, and extensive characterization. The role demands expertise in mammalian and bacterial expression, purification, and analytical biochemistry/biophysics, with collaboration across multidisciplinary teams.

The position is onsite, with opportunities to contribute to AI-assisted workflows and high-throughput analyses, driving

Qualifications

• Bachelor's degree with 2+ years of relevant experience.
• Hands-on experience with recombinant protein expression in both mammalian and bacterial (E. coli) systems.
• Preferred: 4+ years of relevant experience, or a Master's with 2+ years of relevant experience.

Responsibilities

• Execute and optimize recombinant protein expression workflows in mammalian and bacterial (E. coli) expression systems.
• Perform transient mammalian cell culture and transfection for protein production.
• Conduct recombinant protein purification using chromatography-based methods including affinity, ion exchange, and size‑exclusion chromatography utilizing FPLC systems.
• Support development and optimization of expression, purification, and formulation conditions for challenging proteins and complex antigens.
• Characterize proteins using biochemical and biophysical techniques including SDS-PAGE, western blotting, SEC-MALS, DSC, DSF, BLI, Mass Photometry, and flow cytometry‑based approaches.
• Evaluate protein purity, stability, oligomeric state, aggregation, and biomolecular interactions to support candidate assessment and advancement.
• Analyze, interpret, and summarize experimental data and communicate findings through presentations, technical reports, and project team discussions.
• Develop and implement AI‑assisted workflows for high‑throughput protein screening and characterization.
• Leverage AI to accelerate analysis of protein screening datasets and identify high‑performing candidates.
• Collaborate closely with scientists and cross‑functional teams to support project goals, timelines, and deliverables.
• Identify technical challenges and troubleshoot protein expression, purification, and characterization workflows.
• Support implementation and evaluation of new protein production and characterization technologies to improve workflow efficiency.
• Maintain accurate experimental records and support inventory management, reagent tracking, and logistics coordination for partnered programs.

Skills

Protein expression

Mammalian expression

Bacterial expression (E. coli)

Protein purification

Biochemical characterization

Biophysical characterization

Data analysis

Communication skills

Education

Bachelor's degree in Biochemistry, Molecular Biology, Biomedical Science, Protein Engineering, Chemical Biology, or related discipline

Tools

FPLC systems

SDS-PAGE

Western blot

SEC-MALS

DSC

DSF

BLI

Mass Photometry

Job description

Moderna is seeking a Senior Research Associate to join the Protein Design team at its Cambridge, MA site. This role will support protein expression, purification, and biophysical characterization efforts for Infectious Disease Research vaccine and therapeutic programs. The successful candidate will contribute to the discovery and development of vaccine antigens and therapeutic proteins by leveraging expertise in protein biochemistry, molecular biology, and analytical characterization.

The candidate will be responsible for executing protein production workflows in both mammalian and bacterial (E. coli) expression systems, optimizing purification processes, and applying a broad range of biochemical and biophysical techniques to evaluate protein quality, stability, and function. This role requires strong technical expertise, attention to detail, scientific curiosity, and collaboration across multidisciplinary teams to advance multiple research programs in a fast-paced and highly innovative environment.

This is an exciting opportunity to contribute to cutting-edge vaccine and therapeutic development while generating high-quality experimental data that enables critical program decisions.

Here’s What You’ll Do

• Execute and optimize recombinant protein expression workflows in mammalian and bacterial (E. coli) expression systems.
• Perform transient mammalian cell culture and transfection for protein production.
• Conduct recombinant protein purification using chromatography-based methods including affinity, ion exchange, and size‑exclusion chromatography utilizing FPLC systems.
• Support development and optimization of expression, purification, and formulation conditions for challenging proteins and complex antigens.
• Characterize proteins using biochemical and biophysical techniques including SDS-PAGE, western blotting, SEC-MALS, DSC, DSF, BLI, Mass Photometry, and flow cytometry‑based approaches.
• Evaluate protein purity, stability, oligomeric state, aggregation, and biomolecular interactions to support candidate assessment and advancement.
• Analyze, interpret, and summarize experimental data and communicate findings through presentations, technical reports, and project team discussions.
• Develop and implement AI‑assisted workflows for high‑throughput protein screening and characterization.
• Leverage AI to accelerate analysis of protein screening datasets and identify high‑performing candidates.
• Collaborate closely with scientists and cross‑functional teams to support project goals, timelines, and deliverables.
• Identify technical challenges and troubleshoot protein expression, purification, and characterization workflows.
• Support implementation and evaluation of new protein production and characterization technologies to improve workflow efficiency.
• Maintain accurate experimental records and support inventory management, reagent tracking, and logistics coordination for partnered programs.

Here’s What You’ll Need (Basic Qualifications)

• Bachelor's degree in Biochemistry, Molecular Biology, Biomedical Science, Protein Engineering, Chemical Biology, or a related scientific discipline with 2+ years of relevant experience.
• Hands‑on experience with recombinant protein expression in both mammalian and bacterial (E. coli) systems.

Here’s What You’ll Bring to the Table (Preferred Qualifications)

• Bachelor's degree in Biochemistry, Molecular Biology, Biomedical Science, Protein Engineering, Chemical Biology, or a related scientific discipline with 4+ years of relevant experience, or a Master's degree with 2+ years of relevant experience.
• Experience with protein purification workflows and operation of FPLC systems.
• Experience with biochemical and biophysical characterization methods including SDS-PAGE, western blotting, SEC-MALS, DSC, DSF, BLI, Mass Photometry, or related techniques.
• Demonstrated ability to troubleshoot and optimize protein expression, purification, and characterization workflows.
• Experience analyzing protein quality, stability, aggregation, and biomolecular interactions.
• Strong quantitative data analysis and scientific problem‑solving skills with attention to detail.
• Ability to manage multiple experiments and priorities in a collaborative, cross‑functional research environment.
• Excellent written and verbal communication skills.
• A desire to make an impact as part of a high‑growth, transformational company that is Bold, Relentless, Curious, and Collaborative.

Our Working Model

As we build our company, we have always believed that an in‑person culture is critical to our success.

This role is 100% onsite, reflecting the importance of being physically present to support the work, collaborate closely with colleagues, and contribute to our site‑based operations.

Working onsite enables real‑time collaboration, strong team connections, direct mentorship, and the hands‑on partnership needed to deliver our mission. Join us in shaping a world where every interaction is an opportunity to learn, contribute, and make a meaningful impact.

Moderna is a smoke‑free, alcohol‑free, and drug‑free work environment.

Pay & Benefits

At Moderna, we believe that when you feel your best, you can do your best work.

That’s why our benefits and well‑being resources are designed to support you—at work, at home, and everywhere in between.

• Competitive healthcare, plus voluntary benefit programs to support your unique needs
• A holistic approach to well‑being, with access to fitness, mindfulness, and mental health support
• Family planning benefits, including fertility, adoption, and surrogacy support
• Generous paid time off, including vacation, volunteer days, sabbatical, global recharge days, and a discretionary year‑end shutdown
• Savings and investments to help you plan for the future
• Location‑specific perks and extras

The salary range for this role is $74,000.00 - $118,400.00. This is the lowest to highest salary we in good faith believe we would pay for this role at the time of this posting. An individual’s position within the salary range will be based on several factors including, but not limited to, specific competencies, relevant education, qualifications, certifications, experience, skills, performance, and business or organizational needs. The successful candidate may be eligible for an annual discretionary bonus, other incentive compensation, or equity award, subject to company plan eligibility criteria and individual performance.

About Moderna

Since our founding in 2010, we have aspired to build the leading mRNA technology platform, the infrastructure to reimagine how medicines are created and delivered, and a world‑class team. We believe in giving our people a platform to change medicine and an opportunity to change the world.

By living our mission, values, and mindsets every day, our people are the driving force behind our scientific progress and our culture. Together, we are creating a culture of belonging and building an organization that cares deeply for our patients, our employees, the environment, and our communities.

We are proud to have been recognized as a Science Magazine Top Biopharma Employer, a Fast Company Best Workplace for Innovators, and a Great Place to Work in the U.S.

Equal Opportunities

Moderna is committed to equal employment opportunity and non‑discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry or citizenship, ethnicity, disability, military or protected veteran status, genetic information, sexual orientation, marital or familial status, or any other personal characteristic protected under applicable law. Moderna is a place where everyone can grow.

Accommodations

We’re focused on attracting, retaining, developing, and advancing our employees. By cultivating a workplace that values diverse experiences, backgrounds, and ideas, we create an environment where every employee can contribute their best.

Moderna is committed to offering reasonable accommodations to qualified job applicants with disabilities. Any applicant requiring an accommodation in connection with the hiring process and/​or to perform the essential functions of the position for which the applicant has applied should contact the Accommodations team at [e-mail].

Export Control Notice

This position may involve access to technology or data that is subject to U.S. export control laws, including the Export Administration Regulations (EAR). As such, employment is contingent upon the applicant’s ability to access export‑controlled information in accordance with U.S. law. Due to the nature of the work and regulatory requirements, only individuals who qualify as U.S. persons (citizens, permanent residents, asylees, or refugees) are eligible for this position. For this role Moderna is unable to sponsor non‑U.S. persons to apply for an export control license.