Senior Scientist, Antibody Repertoire Sequencing & Discovery

Allergan; Worcester (MA); On-site; USD 120,000 - 180,000; Full time

Job summary

AbbVie Bioresearch Center in Worcester, MA, seeks a Senior Scientist to lead antibody repertoire sequencing efforts and expand RepSeq capabilities for therapeutic discovery in immunology, oncology and neurology.

The role blends wet-lab and computational work, collaborating with data scientists to advance RepSeq analyses for immunology, neurology and oncology programs in a fast-paced, matrixed team.

Qualifications

• PhD or MS/BS with extensive experience in immunology and antibody biology
• Experience with germline genes, V(D)J recombination and SHM
• NGS technologies and platforms including Illumina NextSeq/MiSeq and PacBio
• Molecular biology skills: PCR, cloning, Gibson Assembly
• Ability to streamline processes and maintain documentation
• Excellent communication in a matrixed team

Responsibilities

• Act as expert in RepSeq for therapeutic antibody discovery
• Collaborate with computational scientists to optimize repertoire analysis
• Generate, document and archive repertoire data with metadata
• Analyze results and present at team meetings
• Coordinate work across a matrixed project team
• Demonstrate scientific excellence across multiple projects
• Demonstrate strong interpersonal and communication skills

Skills

Immunoglobulin genetics

RepSeq

Computational biology

Molecular biology

Data analysis

Cross-functional collaboration

Education

PhD in immunology or related

MS in biology or bioinformatics

BS in related field

Tools

PipeBio

Geneious

Sequencher

MS Office

Job description

Company Description

About AbbVie AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com. Follow @abbvie on LinkedIn, Facebook, Instagram, X and YouTube.

Job Description

The Biotherapeutics and Genetics Medicine department within Discovery Research is seeking a highly motivated Senior Scientist to join the Molecular Core team at AbbVie Bioresearch Center (ABC). The ABC site in Worcester, MA, offers a unique setting where Biotherapeutics discovery, development and manufacturing coexist in one location. The Molecular Core partners with multidisciplinary groups across AbbVie focused on developing therapies for Immunology, Neurology and Oncology diseases. We utilize innovative approaches for antibody discovery including in vivo technologies, custom immunization strategies, advanced multiplex screening assays and best-in-class automation platforms to enable successful antibody discovery against complex targets.

As a member of the in vivo antibody discovery group, you will be responsible for optimizing and expanding antibody repertoire sequencing capabilities in antibody discovery, as well as developing innovative methods to help maintain AbbVie’s industry-leading standards. The successful candidate will generate original technical ideas and research strategies for antibody repertoire sequencing (RepSeq) and analysis. They will have technical expertise in generating and applying RepSeq data to therapeutic antibody discovery including computational proficiency enabling highly effective collaboration with computational scientists. This is an exciting opportunity to help drive antibody discovery programs within a multidisciplinary research group in a dynamic, fast-paced environment.

Responsibilities

• Act as scientific and technical expert in antibody repertoire sequencing (RepSeq/AIRR-Seq/BCR-Seq) for therapeutic antibody discovery and hit expansion–Bulk sequencing, 10X, and other commercial and proprietary platforms.
• Collaborate with computational scientists to optimize repertoire analysis and open new avenues for antibody discovery through repertoire guided machine learning and generative design.
• Generate, document, and archive repertoire data with appropriate context and metadata to support broader team access and future utilization.
• Analyze and interpret results, placing data in proper scientific context through review of relevant literature, and present findings at team meetings.
• Regularly present data, communicate effectively, and coordinate work within a highly matrixed project team environment.
• Demonstrate scientific excellence through dedication to multiple projects in a fast-paced, multidisciplinary environment.
• Exhibit a can-do attitude and strong interpersonal and communication skills when working across multiple functional teams.

Qualifications

Education:

• BS, MS, or PhD in immunology, molecular biology, biochemistry, bioinformatics, or a related field with typically 0-4+ (PhD), 8-10+ (MS), or 10-12+ (BS) years of relevant experience.

Required:

• Extensive scientific expertise in immunoglobulin genetics and antibody biology; specifically, the candidate should be well versed in antibody diversity generation (germline genes/V(D)J recombination/somatic hypermutation), affinity maturation, and B cell biology.
• Extensive technical expertise in the isolation and molecular biology of antibody variable region genes for Next-generation Sequencing (NGS) and the capture of RepSeq/AIRR-Seq/BCR-Seq data; both for single-chain and paired heavy- and light-chain recovery.
• Understanding of NGS technologies and platforms – Illumina (NextSeq, MiSeq) and PacBio.
• Understanding and proficiency in molecular biology techniques (e.g. PCR, primer design, molecular cloning, Gibson Assembly, plasmid design).
• Ability to improve and streamline existing processes.
• Strong attention to quality and details when exercising organizational, documentation and communication skills.
• Proven flexibility to deal with changing timelines and shifting work priorities.
• Competency with software programs, including antibody analysis (PipeBio), DNA analysis (Geneious, Sequencher) and MS Office (Excel, PowerPoint, Word).
• Fluency in computational science and familiarity with the application of predictive AI/ML tools and protein language models (PLMs) for antibody discovery.

Desirable:

• Experience in non-human and non-murine immunogenetics.
• Prior experience with bioinformatics and computational analysis of large DNA and protein data sets is highly desired.
• Experience with repertoire sequencing from in vitro antibody discovery libraries.
• Experience with mammalian cell culture, aseptic technique, and transient transfection.
• Experience in immunoassays using techniques like ELISA and flow cytometry.

Position will be filled at level commensurate with extent of education, experience, and accomplishment - Senior Scientist I or Senior Scientist II.

Additional Information

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.
• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.
• This job is eligible to participate in our short-term incentive program.
• This job may be eligible to participate in our long-term incentive program.

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employer remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company’s sole and absolute discretion, consistent with applicable law.

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives and serving our community. Equal Opportunity Employer / Veterans / Disabled.

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