Scientist - Yen Lab/TGEn Group (Hematopoietic Stem Cell, Genome Editing)

Thecentermemphis; Memphis (TN); On-site; USD 86,320 - 154,960; Full time

Benefits offered by this job

Exceptional benefits

Job summary

Thecentermemphis seeks a Scientist to be part of Dr. Jonathan Yen's lab in Memphis, Tennessee. This position focuses on developing genome editing strategies with the goal of treating sickle cell disease.

The ideal candidate will have expertise in hematopoietic stem cells and genome editing technologies, with opportunities to work on critical projects impacting patients globally. Candidates should possess a relevant bachelor's degree, with higher degrees preferred, and significant post-degree experience in research.

Qualifications

• Eight years of relevant post-degree work experience required with a Bachelor’s degree.
• Six years of relevant post-degree work experience required with a Master’s degree.
• Three years of relevant post-degree work experience required with a PhD.

Responsibilities

• Perform studies to develop genome editing strategies for human hematopoietic stem cells.
• Design and execute IND-enabling experiments for genome-edited HSC products.
• Characterize efficacy, safety, and potency of cellular drug products.

Skills

Genome editing

Hematopoietic stem cell biology

Flow cytometry

Experimental method development

Education

Bachelor’s degree in a relevant scientific area

Master’s or PhD degree in a relevant scientific area

Tools

RT-PCR

ddPCR

Next-generation sequencing

Single-cell sequencing

Job description

Overview

The Scientist performs and/or coordinates a wide range of laboratory research procedures and conducts a spectrum of complex support tasks and projects determined by the field and scope of the particular research study. Also, performs a variety of laboratory techniques independently and presents results.

At St. Jude Children’s Research Hospital, talented faculty and staff work together and with colleagues worldwide to advance the research and treatment of pediatric cancer and other catastrophic diseases.

About the Lab

We are seeking an outstanding, highly motivated, and creative Scientist to join Dr. Jonathan Yen's lab and the Therapeutic Genome Engineering (TGEn) group. You will be part of a multidisciplinary team of scientists and clinical researchers helping to advance genome editing strategies for treating sickle cell disease to a Phase 1 clinical trial. Our ongoing therapeutic pipeline is fueled by internal scientific discoveries directed from “bench-to-bedside” and academic collaborations. Come join TGEn to enGENEer concepts to cures!

You will have the opportunity to work with a talented interdisciplinary team of scientists to develop a cellular therapy with the goal of treating patients here at St. Jude Children's Research Hospital and worldwide. The ideal candidate will have a strong combination of experience with genome editing and human hematopoietic stem cells (HSCs).

You will also have the opportunity to learn about and support new, exciting projects, including the development and optimization of pre-clinical genome editing strategies to treat hemoglobinopathies and other hematological diseases, process development, and IND-enabling studies (Pharm/Tox).

Ideal Candidate

The ideal candidate will bring expertise in hematopoietic stem cell biology and genome editing technologies.

Job Responsibilities

• Perform studies to develop genome editing strategies for human hematopoietic stem cells (HSC).
• Design and execute IND-enabling experiments to characterize the pharmacology of a genome-edited human hematopoietic stem cell product to treat sickle cell disease.
• Characterize the efficacy, safety, and potency of the HSC cellular drug product in in vitro and xenotransplantation assays.
• Perform multicolor flow cytometry and FACS to sort hematopoietic populations of relevance.
• Develop experimental methods including RT-PCR, ddPCR, next-generation sequencing, and single-cell sequencing.
• Perform NGS library preparation and analysis of editing outcomes, on-target and off-target.
• Evaluate scale-up and close-system processes to ensure reproducibility of the drug product.
• Conduct exploratory research on developing novel in vitro and in vivo delivery strategies.
• Interact and collaborate with multidisciplinary teams to advance the program.
• Establish tracking and trending analyses of process development and pilot runs.
• Draft documentation (protocols, batch records, reports) to standardize and transfer process to GMP facility.
• Maintain regular and predictable attendance.
• Perform other related duties as assigned to meet the goals of the department and institution.

Minimum Education

• Bachelor’s degree in a relevant scientific area is required.
• Master’s or PhD degree in a relevant scientific area is highly preferred.

Scientist Minimum Experience

• Eight (8) years of relevant post-degree work experience is required with a Bachelor’s degree.
• Six (6) years of relevant post-degree work experience is required with a Master’s degree.
• Three (3) years of relevant post-degree work experience is required with a PhD.
• Experience leading and supporting research experiments preferred.
• Some experience leading lab and scientific project coordination, maintenance, and other research support activities preferred.
• Experience in guiding and training other laboratory personnel preferred.
• Proven performance in earlier role or comparable role.

Compensation

In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience, training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $86,320 - $154,960 per year for the role of Scientist - Yen Lab/TGEn Group (Hematopoietic Stem Cell, Genome Editing).

Benefits

Explore our exceptional benefits.

Equal Opportunity Employer

St. Jude is an Equal Opportunity Employer.