Scientist, Protein Expression

PetsApp; Cambridge (MA); On-site; USD 75,000 - 95,000; Full time

Benefits offered by this job

Competitive salary

Equity

Health insurance

401K

Job summary

PetsApp is looking for a Scientist focused on Protein Expression to join the team in Cambridge. The role involves driving recombinant protein expression in filamentous fungi and requires expertise in molecular cloning and protein characterization techniques. You will work collaboratively with various teams to tackle complex biological challenges and mentor research associates.

A relevant Ph.D. and strong background in molecular biology are essential. The position offers competitive salary and benefits, including health insurance and 401K.

Qualifications

• Ph.D. (or equivalent experience) in biochemistry, bioengineering, microbiology, synthetic biology, or related field.
• Strong molecular cloning skills, with experience constructing complex genetic assemblies.
• Experience with protein expression and characterization including SDS-PAGE, western blot, and ELISA.

Responsibilities

• Design and execute molecular cloning workflows for optimizing fungal expression.
• Optimize genetic systems for genome-wide screening and sequencing.
• Independently troubleshoot experimental challenges and propose solutions.

Skills

Molecular cloning

Protein expression characterization

Scientific communication

Experimental troubleshooting

Education

Ph.D. in relevant field

Tools

SDS-PAGE

Western blot

ELISA

Python

Job description

Scientist - Protein Expression (Full-Time)

Location: Cambridge, MA (On-site)
Company: Anthology Bio

Company summary

Anthology is Boston-based genome design company. We engineer fungi through advanced genetics, microfluidics, and AI to biomanufacture functional proteins at 5-10x lower cost compared to extracting these proteins from their natural sources. Our goal is to build a climate-resilient protein supply chain for human and planetary health. Backed by leading deep-tech investors, we have partnered with global nutrition and ingredient manufacturers to bring cost-effective proteins to market.

Overall:

We are looking for a scientist to drive recombinant protein expression in filamentous fungi. Reporting to the Director of R&D, you will own core platform work - from molecular cloning and fungal transformation through expression screening and assay development - while also contributing to genome-wide screening and sequencing efforts. You will mentor research associates, collaborate across microfluidics and computational teams, and bring both rational and evolutionary engineering approaches to bear on hard expression problems. This is a role for someone who can operate independently, troubleshoot creatively, and grow with the platform.

Responsibilities:

• Design and execute molecular cloning workflows to construct and optimize fungal expression

• Optimize genetic systems for genome-wide screening and sequencing

• Develop and run protein expression assays in shake flask format, from culture setup through quantification and characterization

• Independently troubleshoot experimental challenges, propose solutions, and develop improved experimental plans

• Communicate and collaborate effectively with team members across diverse scientific and technical roles

• Manage and mentor research associates with varied scientific backgrounds

• Work with the microfluidics team to adapt and validate protein assays for microdroplet-based high-throughput screening

• Maintain rigorous experimental documentation to support reproducibility and platform iteration

Qualifications:

• Ph.D. (or equivalent experience) in biochemistry, bioengineering, microbiology, synthetic biology, systems biology, or a related field

• Strong molecular cloning skills and experience constructing complex genetic assemblies

• Experience with protein expression and characterization including SDS-PAGE, western blot, ELISA, mass spec, or equivalent assays

• Strong understanding of rational and evolutionary engineering principles

• Able to work on-site in Cambridge, MA

• Authorized to work in the United States (STEM-OPT or J1 are welcomed)

Nice to have

• Direct experience culturing and/or engineering filamentous fungi (Aspergillus,Trichoderma, or similar) and/or yeast (S. cerevisiae, Pichia pastoris, or similar)

• Familiarity with protein design using biological language models (ESM, ProteinMPNN, RFdiffusion, or similar)

• Python scripting experience - data processing, assay analysis, or bioinformatics workflows

Why work at Anthology?

• Work on a genuinely hard, open-ended problem - fungal expression biology at the frontier of what's commercially relevant

• Collaborate across wet lab, microfluidics, and computational teams in a highly integrated environment

• Brand-new 150K sq ft deep-tech facility at The Engine by MIT

• Competitive salary, equity, and benefits (health insurance, 401K)

Start Date:

ASAP