Scientist - II

Artech Information System LLC; Spring House (PA); On-site; USD 80,000 - 100,000; Full time

Job summary

A leading IT staffing firm is seeking a candidate to engineer stably-transfected T cell lines for treating hematological malignancies. Candidates are expected to perform extensive cell culture methodologies and analyses, while maintaining collaboration within a scientific team. A master's degree in a relevant field with at least 2 years of industry experience is required. Preferred candidates will have knowledge of primary T cells, electroporation, and gene editing techniques. This is an opportunity to contribute to significant advancements in medical technology.

Qualifications

• M.S. degree in Biomedical Engineering, Molecular Biology, Cell Biology, Biochemistry, Chemistry or related field.
• 2+ years of industry experience is required.
• Experience with primary T cells and gene editing is preferred.

Responsibilities

• Engineer multiple stably-transfected T cell lines.
• Perform extensive cell culture methodologies and downstream analyses.
• Maintain experimental data in an electronic laboratory notebook.

Skills

Mammalian cell culture

Gene delivery platforms

Flow cytometry

Downstream functional validation

Education

M.S. in Biomedical Engineering or related field

Tools

Electroporation platforms

CRISPR

Multiplex ELISA

Viral-delivery techniques

Job description

Artech is the 10th Largest IT Staffing Company in the US, according to Staffing Industry Analysts' 2012 annual report. Artech provides technical expertise to fill gaps in clients' immediate skill-sets availability, deliver emerging technology skill-sets, refresh existing skill base, allow for flexibility in project planning and execution phases, and provide budgeting/financial flexibility by offering contingent labor as a variable cost.

Job Description

The candidate will contribute to the production and functional assessment of chimeric antigen receptor T cells for the treatment of hematological malignancies. She/he will work to optimize multiple nucleic-acid delivery platforms for both primary and established T cell lines for the purpose of generating stable transfectants. The scientist will subsequently characterize the short- and long-term functionality of the product(s) via an array of cell-based platforms.

ESSENTIAL FUNCTIONS:

• The candidate will work to engineer multiple stably-transfected T cell lines using both mRNA- and pDNA-based delivery platforms. This effort will encompass extensive cell culture methodologies, as well as the ability to perform downstream analyses necessary to validate delivery of the transgene(s).
• A diverse background in mammalian cell culture is absolutely essential.
• Experience with primary T cells is preferred. The successful applicant should demonstrate extensive knowledge surrounding cell culture techniques, gene delivery platforms (i.e., transfection/transduction), and downstream functional validation of resultant cells.
• Experience with mammalian cell-based electroporation platforms (i.e., Amaxa, MaxCyte, BTX, etc.) is required. Preference will be given to candidates with expertise in flow cytometry, gene editing (i.e., CRISPR, transposase, etc.), multiplex ELISA, and viral-delivery techniques (i.e., AAV, lentivirus).
• The successful candidate will work independently, but cooperatively, within the larger group. The ability to generate and maintain experimental data in an electronic laboratory notebook in a highly organized manner is essential.

Qualifications

Education:

• M.S. in Biomedical Engineering, Molecular Biology, Cell biology, Biochemistry, Chemistry or a related field with 2+ years industry experience.