Research Technician - Makino Lab

University of Florida; Florida; Vor Ort; USD 23.000 - 32.000; Vollzeit

Zusammenfassung

The University of Florida Makino Lab in Palm Beach is seeking a Research Technician to support translational cardiovascular research. The role centers on mouse colony management, basic molecular biology, and functional assessments using echocardiography or the Millar catheter.

You will collaborate with a research team, maintain meticulous records, perform PCR/qPCR/WB and cell culture, and troubleshoot workflows to ensure high-quality scientific outcomes.

Qualifikationen

• Bachelor's degree in an appropriate area of specialization or an equivalent combination of education and experience.
• Experience with mouse colony maintenance including genotyping-related tasks.
• Hands-on experience with molecular biology techniques (PCR, qPCR, WB) and cell culture.
• Aseptic technique, accurate record-keeping, and lab organization.

Aufgaben

• Maintain mouse colonies including weekly checks, mating, weaning, and tailing for genotyping.
• Perform basic molecular biology experiments (PCR/qPCR/WB) and cell culture under sterile conditions.
• Conduct animal experiments and assess cardiac/vascular functions via echocardiography or Millar catheter.
• Coordinate with lab members, maintain equipment/reagents, and manage research records.
• Perform data acquisition and analysis.

Kenntnisse

Mouse colony maintenance

PCR

qPCR

Western blot

Cell culture

Aseptic techniques

Data analysis

Team collaboration

Genotyping

Ausbildung

Bachelor's degree

Tools

GraphPad

EndNote

MS Word/Excel/PowerPoint

Jobbeschreibung

Research Technician - Makino Lab

Job no: 541858
Work type: Staff Full-Time
Location: Palm Beach
Categories: Biology/Life Science, Grant or Research Administration, Veterinary Medicine/Animal Care
Department: 37046020 - SR-CISSM-MAKINO LAB

Classification Title: Research Technician

Classification Minimum Requirements: Bachelor's degree in an appropriate area of specialization or an equivalent combination of education and experience.

Dr. Ayako Makino's laboratory is seeking a Research Technician to join our translational research team, specializing in cardiovascular pathophysiology and vascular cell biology. Our research focuses on the pathogenic mechanisms of coronary vascular complications in diabetes and menopause, as well as the pathobiology of pulmonary hypertension. The major tasks for this position are 1) maintaining mouse colonies (i.e., weekly checking, mating, weaning, tailing, genotyping, chemical administration), and 2) assessment of cardiac and vascular functions using an echocardiogram and/or a Millar catheter. The incumbent must possess skills to handle small animals (i.e., holding, administering chemicals, dissection of tissues), basic molecular biological skills (e.g., PCR, WB, qPCR, cell culture), precise record-keeping skills, aseptic techniques, organizational skills, and the ability to work collaboratively with other lab members. Additionally, the role involves troubleshooting technical issues, analyzing data, and contributing to the efficient functioning of the lab to facilitate high-quality scientific outcomes.

Responsibilities and Duties:

• Maintain our mouse colonies by conducting weekly checks, cleaning cages, weaning litter, mating, and tailing for genotyping.
• Perform basic molecular biological experiments, including PCR for genotyping, qPCR, WB, as well as culturing cells under aseptic conditions.
• Conduct animal experiments, such as administration of chemicals and assessment of cardiac and vascular functions using echocardiography and/or the Millar catheter.
• Coordinate with other investigators for lab research, including maintaining the function of lab equipment and reagents and managing the lab research record (including methodological approaches and animal protocols).
• Perform data acquisition and analysis.

Expected Salary: $20.00 per hour.

Required Qualifications: Bachelor's degree in an appropriate area of specialization or an equivalent combination of education and experience.

Preferred:

• Demonstrated ability to perform mouse colony maintenance, including checking mice, cleaning cages, mating, weaning, and tailing
• Demonstrated skills in animal experiments, including chemical administration and assessment of cardiac and vascular functions using echocardiography
• Demonstrated skills in precise record-keeping
• Theoretical and working knowledge in molecular biology, including PCR, qPCR, and WB
• Aseptic techniques for cell culture.
• Demonstrated ability to conduct data analysis and interpretation of the results, and troubleshoot.
• Demonstrated effective time management skills to plan and organize work assignments in an efficient and productive manner.
• Demonstrated ability to work as a team member and coordinate with others to maintain lab functionality.
• Demonstrated knowledge of the following specialized programs: GraphPad, EndNote, and MS Word/Excel/PowerPoint.
• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

Health Assessment Required: Yes

This position is eligible for veterans' preference.

The University of Florida is a member of the State University System of Florida and an Equal Opportunity Employer. All qualified applicants will receive consideration for employment based on merit.

If an accommodation due to a disability is needed to apply for this position, please call 352-392-2477 or the Florida Relay System at 800-955-8771 (TDD) or visit Accessibility at UF.

Advertised: 28 Oct 2026 Eastern Daylight Time
Applications close: Open until filled