Research Technician - Makino Lab

University of Florida; Florida; In loco; USD 23.000 - 32.000; Tempo pieno

Descrizione del lavoro

The University of Florida in Palm Beach, FL, is seeking a Research Technician to join Dr. Ayako Makino’s translational research team.

The role focuses on cardiovascular pathophysiology and vascular cell biology, maintaining mouse colonies and performing molecular biology experiments to advance high-quality scientific outcomes. The position emphasizes genotyping, echocardiography/Millar catheter-based assessments, aseptic cell culture, and meticulous data interpretation, with a collaborative lab

Competenze

• Bachelor’s degree in an appropriate field or equivalent experience.
• Experience maintaining mouse colonies and performing genotyping-related tasks.
• Experience performing molecular biology techniques (PCR, qPCR, WB) and cell culture basics.
• Strong record-keeping, aseptic technique, and data analysis skills.
• Ability to work in a team and troubleshoot lab issues.

Mansioni

• Maintain mouse colonies by weekly checks, cage cleaning, weaning, mating, and tailing for genotyping.
• Perform basic molecular biology experiments (PCR, qPCR, WB) and cell culture under aseptic conditions.
• Conduct animal experiments including chemical administration and assessment of cardiac/vascular function.
• Coordinate with investigators and maintain lab equipment, reagents, and records.
• Perform data acquisition and analysis.

Conoscenze

Mouse colony maintenance

Animal handling

Molecular biology basics

PCR/qPCR/WB basics

Aseptic techniques

Data analysis

Record-keeping

Teamwork

Time management

Willingness to learn

Formazione

Bachelor's degree in appropriate field

Strumenti

GraphPad

EndNote

MS Word/Excel/PowerPoint

Descrizione del lavoro

Job no: 541858
Work type: Staff Full-Time
Location: Palm Beach
Categories: Biology/Life Science, Grant or Research Administration, Veterinary Medicine/Animal Care
Department: 37046020 - SR-CISSM-MAKINO LAB

Classification Title:

Research Technician

Bachelor's degree in an appropriate area of specialization or an equivalent combination of education and experience.

Job Description:

Dr. Ayako Makino’s laboratory is seeking a Research Technician to join our translational research team, specializing in cardiovascular pathophysiology and vascular cell biology. Our research focuses on the pathogenic mechanisms of coronary vascular complications in diabetes and menopause, as well as the pathobiology of pulmonary hypertension. The major tasks for this position are 1) maintaining mouse colonies (i.e., weekly checking, mating, weaning, tailing, genotyping, chemical administration), and 2) assessment of cardiac and vascular functions using an echocardiogram and/or a Millar catheter. The incumbent must possess skills to handle small animals (i.e., holding, administering chemicals, dissection of tissues), basic molecular biological skills (e.g., PCR, WB, qPCR, cell culture), precise record-keeping skills, aseptic techniques, organizational skills, and the ability to work collaboratively with other lab members. Additionally, the role involves troubleshooting technical issues, analyzing data, and contributing to the efficient functioning of the lab to facilitate high-quality scientific outcomes.

Responsibilities and Duties:

• Maintain our mouse colonies by conducting weekly checks, cleaning cages, weaning litter, mating, and tailing for genotyping.
• Perform basic molecular biological experiments, including PCR for genotyping, qPCR, WB, as well as culturing cells under aseptic conditions.
• Conduct animal experiments, such as administration of chemicals and assessment of cardiac and vascular functions using echocardiography and/or the Millar catheter.
• Coordinate with other investigators for lab research, including maintaining the function of lab equipment and reagents and managing the lab research record (including methodological approaches and animal protocols).
• Perform data acquisition and analysis.

$20.00 per hour.

Required Qualifications:

Required Qualifications: Bachelor's degree in an appropriate area of specialization or an equivalent combination of education and experience.

Preferred:

• Demonstrated ability to perform mouse colony maintenance, including checking mice, cleaning cages, mating, weaning, and tailing
• Demonstrated skills in animal experiments, including chemical administration and assessment of cardiac and vascular functions using echocardiography
• Demonstrated skills in precise record-keeping
• Theoretical and working knowledge in molecular biology, including PCR, qPCR, and WB
• Aseptic techniques for cell culture.
• Demonstrated ability to conduct data analysis and interpretation of the results, and troubleshoot.
• Demonstrated effective time management skills to plan and organize work assignments in an efficient and productive manner.
• Demonstrated ability to work as a team member and coordinate with others to maintain lab functionality.
• Demonstrated knowledge of the following specialized programs: GraphPad, EndNote, and MS Word/Excel/PowerPoint.
• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

This position is eligible for veterans’ preference.

Work schedule: Monday – Friday. 40 hours per week. Need to be available for the weekend (occasional) for chronic injection/treatment/cytology as part of mouse colony maintenance work. No hybrid schedule will be necessary.

This is a time-limited position.

This position is open until filled. Application review will begin November 4, 2026.

The University of Florida is a member of the State University System of Florida and an Equal Opportunity Employer. All qualified applicants will receive consideration for employment based on merit.

If an accommodation due to a disability is needed to apply for this position, please call 352-392-2477 or the Florida Relay System at 800-955-8771 (TDD) or visit Accessibility at UF .

Health Assessment Required:

Health Assessment Required: Yes

Advertised: 28 Oct 2026 Eastern Daylight Time
Applications close: Open until filled

The University of Florida is an Equal Employment Opportunity Employer.

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