Research Scientist/Engineer 1

University of Washington; Seattle (WA); On-site; USD 62,000 - 84,000; Full time

Job summary

University of Washington in Seattle is seeking a Research Scientist to advance a CAR-T cell project focused on de novo miniprotein inhibitors of tumor enzymes. You will work with graduate students, postdocs, and staff across the King Lab to drive protein design, expression, and functional validation for potential clinical testing.

The role emphasizes hands-on molecular biology, biochemistry, and biophysics, including high-throughput assays, structural biology prep, and data-driven reporting in a

Qualifications

• Bachelor's degree in Biochemistry, Biology or other related field and one year of relevant experience with hands-on experience with standard molecular biology techniques (cloning, PCR, mutagenesis) and protein biochemistry (expression, purification, quantification)
• Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Responsibilities

• Perform recombinant protein expression (bacterial and/or mammalian systems) and purification of de novo designed miniprotein inhibitors
• Assist in the development of a high-throughput, cell-based assay for screening miniprotein libraries
• Engineer CAR-T cells and assess viability and in vitro cytotoxicity
• Conduct biophysical and biochemical assays to characterize protein-protein interactions including SPR/BLI
• Support structural biology efforts, including sample preparation for x-ray crystallography and/or cryo-EM, in coordination with the IPD Research Core Laboratories.
• Assist with literature and structural database (PDB) mining to catalogue relevant structures and binding partner data
• Maintain accurate, organized laboratory records/notebooks and contribute to data management and reproducibility standards-10%
• Assist with data analysis, figure preparation, and presentation of results at lab meetings
• Prepare reagents, buffers, and maintain general lab operations-5%
• Perform other duties as assigned to support the goals of the research program-5%

Skills

Molecular biology

Cloning

PCR

Mutagenesis

Protein expression

Protein purification

Protein quantification

CAR-T cell handling

Biophysical assays

Miniprotein design

Education

Bachelor's degree in Biochemistry, Biology or related field

Tools

SPR

BLI

X-ray crystallography

Cryo-EM

PDB

AlphaFold 3

Job description

Job Description

Seattle, WA

Full time

REQ-0000138108

Field of research:

The Institute for Protein Design (IPD) at the University of Washington is a world-leading research center focused on creating new proteins that solve challenges in medicine, technology, and sustainability. Founded by Nobel laureate David Baker, the IPD fosters a collaborative, interdisciplinary environment, bringing together expertise in computational design, biochemistry, genome sciences, and structural biology.

This position is based in the King Lab at the IPD, a highly collaborative, interdisciplinary research environment spanning computational and wet-lab protein design with biomedical applications.

Purpose of the research project(s):

The King Lab is seeking a motivated Research Scientist to play a critical role in supporting and advancing a project developing the next-generation of chimeric antigen receptor (CAR)-T cell therapies. This project will specifically focus on augmenting the cytotoxic machinery of CAR-T cells by developing de novo miniprotein inhibitors of critical tumor-specific enzymes. If successful, this project will result in a clinical product for testing in first-in-human clinical trials within the UW Cancer Consortium.

The Research Scientist will work closely with graduate students, postdocs, and staff scientists across the King Lab as well as collaborating labs.

Major duties and responsibilities:

Molecular and cell biology-80%

• Perform recombinant protein expression (bacterial and/or mammalian systems) and purification of de novo designed miniprotein inhibitors
• Assist in the development of a high-throughput, cell-based assay for screening miniprotein libraries
• Engineer CAR-T cells and assess viability and in vitro cytotoxicity
• Conduct biophysical and biochemical assays to characterize protein-protein interactions including SPR/BLI
• Support structural biology efforts, including sample preparation for x-ray crystallography and/or cryo-EM, in coordination with the IPD Research Core Laboratories.
• Assist with literature and structural database (PDB) mining to catalogue relevant structures and binding partner data
• Maintain accurate, organized laboratory records/notebooks and contribute to data management and reproducibility standards-10%
• Assist with data analysis, figure preparation, and presentation of results at lab meetings
• Prepare reagents, buffers, and maintain general lab operations-5%
• Perform other duties as assigned to support the goals of the research program-5%

Requirements:

Bachelor's degree in Biochemistry, Biology or other related field and one year of relevant experience with Hands-on experience with standard molecular biology techniques (cloning, PCR, mutagenesis) and protein biochemistry (expression, purification, quantification)

Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Additional requirements:

• Strong scientific problem-solving skills and ability to multitask
• Strong organizational skills, attention to detail, and ability to maintain clear records
• A collaborative mindset with a strong desire to work in a fast-paced, interdisciplinary research environment
• Hands-on experience with handling CAR-T cells or native T cells including production, culture, and in vitro cytotoxicity assays.

Desired:

• At least 2 years of prior experience in an academic or biotech research lab setting
• Experience with biophysical characterization techniques (SPR, BLI)
• Familiarity with structural biology methods
• Experience with Protein Data Bank (PDB) and/or Alpha Fold 3

Compensation, Benefits and Position Details

Pay Range Minimum:

$62,400.00 annual

Pay Range Maximum:

$84,000.00 annual

Other Compensation:

-

Benefits:

For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-uw-staff/

Shift:

First Shift (United States of America)

Temporary or Regular?

This is a regular position

FTE (Full-Time Equivalent):

100.00%

Union/Bargaining Unit:

UAW Research

About the UW

Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world.

UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.

Our Commitment

The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81 (https://policy.uw.edu/directory/po/executive-orders/eo-81-prohibiting-discrimination-harassment-and-sexual-misconduct/) .

To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [e-mail] .

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law (https://app.leg.wa.gov/RCW/default.aspx?cite=28B.112.080) .

University of Washington is an affirmative action and equal opportunity employer.

All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.