Research Lab Specialist I

Children's Hospital of Philadelphia; United States; On-site; USD 58,000 - 73,000; Full time

Job summary

Children's Hospital of Philadelphia (CHOP) seeks a highly skilled researcher to lead stem cell culture and neural differentiation projects focused on neurodevelopmental disorders. You will work with human embryonic stem cells and induced pluripotent stem cells, integrating fluorescent reporters and advanced imaging techniques.

The role emphasizes independent, self-directed work with guidance to junior staff, data analysis, and collaboration across CHOP's Omics Initiative and core facilities.

Qualifications

• Bachelor's degree in a scientific field.
• Minimum 3 years of directly relevant post-grad lab experience.
• Preferred 5+ years of directly relevant post-grad lab experience.
• Advanced knowledge of a wide range of lab techniques and equipment.

Responsibilities

• Maintain, propagate, and QC check human embryonic stem cell and induced pluripotent stem cell lines.
• Modify lines to select stable integrants of fluorescent reporters for synaptic imaging.
• Design and perform assays to study mutational and environmental influences on microglial function.
• Support transcriptomic and genomics studies, including RNA-sequencing sample prep and data organization.
• Analyze results, troubleshoot experiments, and contribute to manuscripts.
• Collaborate with researchers across neural differentiations and CHOP Omics Initiative.
• Maintain inventory and assist with lab safety and protocols.
• May supervise junior staff and assist with lab administration functions.

Skills

Lab techniques

Scientific software

Verbal & written comms

Organizational skills

Critical thinking

Analytical skills

Education

Bachelor's degree

Job description

SHIFT: Day (United States of America)

Seeking Breakthrough Makers

Children’s Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation. At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care—and your career. CHOP does not discriminate on the basis of race, color, sex, national origin, religion, or any other legally protected categories in any employment, training, or vendor decisions or programs. CHOP recognizes the critical importance of a workforce rich in varied backgrounds and experiences and engages in ongoing efforts to achieve that through equally varied and non-discriminatory means.

A Brief Overview

This role participates in a planned research projects by performing research protocols. Works independently and self-directed while providing guidance and technical training to other lab staff. Possesses a specialized education in the biological or chemical sciences and is required to exercise appropriate situational discretion and sound scientific judgement in execution / interpretation of experiments, and interpretation of data derived.

This role is well suited for a researcher who enjoys human stem cell culture (hESCs and iPSCs), their modification with fluorescent reporters of synaptic activity, and their differentiation into excitatory and inhibitory neurons, astrocytes, blood brain barrier endothelium, and especially brain macrophages/microglia. The researcher should also enjoy applying that expertise to biologically and clinically relevant questions--with emphasis on neurodevelopmental disorders such as intellectual disability, autism, and schizophrenia. The successful candidate will have opportunities to build advanced skills in cell culture, immunofluorescence, microscopy, disease modeling, and collaborative research while contributing directly to projects with the potential to uncover disease mechanisms and identify therapeutic strategies.

What you will do

• Maintain, propagate, and QC check human embryonic stem cell and induced pluripotent stem cell lines.
• Modify lines the select for stable integrants of fluorescent reporters for synaptic imaging.
• Help design and perform assays to study various mutational and environmental influences on microglial function, including in mixed differentiations with human neurons and astrocytes and including xenotransplantations into genetically modified mice.
• Support transcriptomic and genomics studies, including RNA-sequencing sample preparation, data organization, and coordination with genomics and bioinformatics cores.
• Analyze results, troubleshoot experiments, prepare figures and presentations, and contribute to manuscripts.
• Collaborate with researchers working in neuronal differentiations, the CHOP Omics Initiative, institutional core facilities, and other project partners while supporting general laboratory operations and student training.
• Performs lab tests which require the analysis of results and interpretation of methods
• Assists with planning specific research protocols and procedures
• Assists in development & troubleshooting of new techniques and ideas
• Modifies methods and protocols under the direction of the PI
• Formulates and performs complex lab experiments, analyzes data & reports outcomes
• Presents & discusses work at regular lab meetings
• Prepares laboratory reagents and monitors and maintains inventory
• Performs calculations and maintains appropriate log books and lab notebooks
• Assists in writing methods and setting-up protocols accurately under the direction of the PI program director or senior scientist
• Assists with the maintenance of laboratory equipment, development of safety procedures and compliance records
• Researches journals, white papers and other scientific publications for pertinent information
• Assists with the preparation of materials for presentations, papers, grant applications, and conferences
• May oversee junior staff and/or laboratory workflow.
• May assist with lab administrative functions.

Education Qualifications

• Bachelor's Degree Required

Experience Qualifications

• At least three (3) years of directly relevant post grad scientific lab experience Required
• At least five (5) years of directly relevant post grad scientific lab experience Preferred

Skills and Abilities

• Advanced knowledge in the use of a wide variety of lab techniques, research equipment and research related software
• Excellent verbal and written communications skills
• Excellent organizational skills
• Excellent critical thinking / problem-solving skills
• Excellent analytical skills

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must receive an annual influenza vaccine. Learn more. EEO / VEVRAA Federal Contractor | Tobacco Statement

SALARY RANGE:

$58,300.00 - $72,900.00 Annually

Salary ranges are shown for full-time jobs. If you're working part-time, your pay will be adjusted accordingly.

At CHOP, we are committed to fair and transparent pay practices. Factors such as skills and experience could result in an offer above the salary range noted in this job posting. Click here for more information regarding CHOP's Compensation and Benefits.