Research Assistant Senior

Meharry Medical College; Nashville (TN); On-site; USD 42,000 - 65,000; Full time

Job summary

Meharry Medical College in Nashville seeks a Research Assistant II to support wetlab operations, including protein, RNA, and DNA work, cell culture, and buffer preparation. The role requires independent work and teamwork, clear communication, strong organization, and meticulous attention to detail.

Responsibilities include maintaining trypanosome and mammalian cultures, protein and DNA analyses, transfection, protocol updates, reagent testing, data assembly for reports, and equipment upkeep.

Qualifications

• Clear communication, organization, and attention to detail are essential.
• Protein expression and purification capabilities are required.
• Familiarity with DNA and protein gel analysis and Western blot is expected.

Responsibilities

• Maintain trypanosome and mammalian cell cultures.
• Perform protein and DNA analysis.
• Prepare buffers and solutions according to protocols.
• Transfect cells and conduct other experiments as instructed.
• Write and update laboratory protocols.

Skills

Protein expression

Purification

DNA gel analysis

Western blot

Data presentation

Protocol following

Communication

Attention to detail

Teamwork

Education

B.Sc. with at least 3 yrs laboratory experience

Tools

Centrifuges

Incubated shakers

Job description

The key role of the RA II will be to perform administrative, research, and technical duties for the laboratory. Primary responsibility will be to support many aspects of well-functioning wetlab, including production and analysis of proteins, RNA, and DNA, cell culture, preparation of buffers and solutions, and other essential tasks. The ability to function independently, and as part of a team, is critical. Must communicate clearly, be organized, and have attention to detail.

Essential Functions (Principal Duties and Job Responsibilities):

Maintain trypanosome and mammalian cell cultures, Protein and DNA analysis, preparation of bacteriological plates, transfection, and performing other experiments as instructed.Writing and updating laboratory protocols. Test and evaluate quality of reagents.Assemble research data for presentation and/or reportsMaintain laboratory equipment including centrifuges, incubated shakersOrdering reagents, keeping inventoryPreparation of buffers and reagents according to the protocolSupervision of graduate/undergraduate students;Performs other related duties as assigned.

Knowledge, Skills and Abilities Required:

Protein expression/purificationFamiliarity with DNA and Protein gel and Western blot analysisQuantitation of DNA and ProteinsFollowing a written protocolPresent data

Education and Experience Required:

B.Sc. with at least 3 yrs laboratory experiences