Research Assistant I Lab

Harvard University; Cambridge (MA); On-site; USD 42,000 - 70,000; Full time

Benefits offered by this job

Generous paid time off

Medical, dental, vision coverage

Tuition assistance and reimbursement

Job summary

Harvard University’s Wagers Laboratory in Cambridge, MA is seeking a motivated lab assistant to support CRISPR/Cas9 genome editing, AAV-based gene transfer, and mouse work. You will assist in cell culture, flow cytometry, immunostaining, and data analysis, while helping with general lab operations and meetings.

The role involves routine animal husbandry, scheduling, and preparation of reagents; regular progress reports will be presented to the PI and team, with supervision as needed.

Qualifications

• At least one year of related lab experience is required.
• Experience with gene editing systems or tissue biology is preferred.
• Ability to operate standard lab equipment and software tools.

Responsibilities

• Perform experiments using AAVs and CRISPR/Cas9 genome editing.
• Manage data and present results in group meetings.
• Maintain lab supplies and assist with genotyping and tissue work.
• Collaborate with team on experimental design and protocols.
• Ensure safety, proper documentation, and equipment upkeep.

Skills

CRISPR/Cas9

AAV techniques

Flow cytometry

Microscopy

Tissue histology

Data presentation

Lab safety

Communication

Tools

CRISPR/Cas9

AAV

Flow cytometer

Microscopes

Cell culture

Job description

Company Description

By working at Harvard University, you join a vibrant community that advances Harvard's world-changing mission in meaningful ways, inspires innovation and collaboration, and builds skills and expertise. We are dedicated to creating a diverse and welcoming environment where everyone can thrive.

Company Description

By working at Harvard University, you join a vibrant community that advances Harvard's world-changing mission in meaningful ways, inspires innovation and collaboration, and builds skills and expertise. We are dedicated to creating a diverse and welcoming environment where everyone can thrive.

Why join the Harvard Faculty of Arts and Sciences?

The Faculty of Arts and Sciences (FAS) is the historic heart of Harvard University. It is the home of Harvard’s undergraduate program (Harvard College, founded in 1636) as well as all of Harvard’s Ph.D. programs (the Harvard Kenneth C. Griffin Graduate School of Arts and Sciences, founded in 1872), Harvard Athletics and the Division of Continuing Education. The 40 academic departments and 30+ centers of the FAS support a community unparalleled in its academic excellence across the broadest range of liberal arts and sciences disciplines. Together, the FAS seeks to foster an environment of ambition, curiosity and shared commitment to knowledge and truth that elicits excellence from all members of our community and prepares the next generation of leaders through a transformative educational experience.

Job Description

Job Summary

The Wagers laboratory studies the biology and function of adult tissue-specific stem cells, particularly those stem cells that form the skeletal muscle and blood. Current topics under investigation include regulation of stem cell function in normal tissues and under abnormal conditions such as aging and cancer, and methods for editing stem cell genomes using CRISPR/Cas9 for experimental and therapeutic purposes. The lab is seeking highly motivated candidates to assist in ongoing experiments that include the development and application of novel CRISPR/Cas9 and adeno-associated virus (AAV) gene transfer technologies, mouse work (colony maintenance, husbandry, genotyping, physiological assessment, tissue harvest, etc.), cell culture, flow cytometry, immunostaining, and bioassays of skeletal muscle and hematopoietic cell function. The assistant will also perform a variety of basic and general lab support and clerical tasks, including ordering and stocking of supplies, maintenance of equipment and preparation and storage of shared reagents. They will also be expected to participate in and present results for regular research group meetings and to contribute to the preparation and presentation of written and oral progress reports, as needed. Work will be performed under the specific direction of the PI, research manager and/or senior lab members, although certain research-related tasks will be performed independently, within the guidelines of and subject to review by the PI and research staff.

Job-Specific Responsibilities

• Performs laboratory experiments utilizing AAVs and CRISPR/Cas9 genome editing complexes, gene engineered mouse models, cell culture, flow cytometry, immunostaining, microscopy, molecular biology and bioassays;
• Collects and safeguards project data; keeps detailed, accurate and up-to-date notes detailing experimental design and results;
• Under direction, processes, organizes and summarizes data, reporting experiment results in written form and in oral presentations, using a variety of scientific, word processing, spreadsheet and statistical software applications or program platforms;
• As a member of a project team, assists in the design and execution of laboratory experiments, techniques, and protocols;
• Performs routine logging and/or testing of samples; routine animal husbandry and experimentation, and genotyping
• May occasionally instruct others in basic laboratory techniques or procedures;
• Performs related laboratory maintenance such as maintaining and cleaning equipment, scheduling repairs, tracking inventory and ordering supplies.

Working Conditions and Physical Requirements

• May be required to lift, move and transport related laboratory equipment.
• Required to work with a variety of hazardous materials under approved safety protocols.
• Required to work with and handle animals.
• This position is based in Cambridge, MA

Qualifications

Basic Qualifications

At least one year related work experience (relevant course work may count towards experience)

Additional Qualifications and Skills

Previous undergraduate or post-graduate lab experience, particularly with gene editing systems, animal models and tissue histology will be preferred. Demonstrated abilities in relevant laboratory techniques is also preferred. Exposure to applicable computer technologies, including specific software applications, is encouraged. Appropriate organizational and communications skills required.

Additional Information

• Appointment End Date: This is a one year grant funded term position with the possibility of extension based on availability of continued funding and satisfactory performance.
• Standard Hours/Schedule: 35 hours per week
• Visa Sponsorship Information: Harvard University is unable to provide visa sponsorship for this position
• Pre-Employment Screening: Identity
• Other Information:
  • All formal offers will be made by FAS Human Resources

Work Format Details

This position has been determined by school or unit leaders that all duties and responsibilities must be performed at a Harvard or Harvard-designated location. Certain visa types may limit work location. Individuals must meet work location sponsorship requirements prior to employment.

Salary Grade and Ranges

This position is salary grade level 052. Please visit Harvard's Salary Ranges to view the corresponding salary range and related information.

Benefits

Harvard offers a comprehensive benefits package that is designed to support a healthy work-life balance and your physical, mental and financial wellbeing. Because here, you are what matters. Our benefits include, but are not limited to:

• Generous paid time off including parental leave
• Medical, dental, and vision health insurance coverage starting on day one
• Retirement plans with university contributions
• Wellbeing and mental health resourcesSupport for families and caregivers
• Professional development opportunities including tuition assistance and reimbursement
• Commuter benefits, discounts and campus perks

Learn more about these and additional benefits on our Benefits & Wellbeing Page.

EEO/Non-Discrimination Commitment Statement

Harvard University is committed to equal opportunity and non-discrimination. We seek talent from all parts of society and the world, and we strive to ensure everyone at Harvard thrives. Our differences help our community advance Harvard's academic purposes.

Harvard has an equal employment opportunity policy that outlines our commitment to prohibiting discrimination on the basis of race, ethnicity, color, national origin, sex, sexual orientation, gender identity, veteran status, religion, disability, or any other characteristic protected by law or identified in the university's non-discrimination policy. Harvard's equal employment opportunity policy and non-discrimination policy help all community members participate fully in work and campus life free from harassment and discrimination.