Postdoctoral fellow

Johns Hopkins University School of Medicine; Baltimore (MD); On-site; USD 54,000 - 68,000; Full time

Job summary

Johns Hopkins University School of Medicine's Na Lab invites applications for a postdoctoral fellow to advance mass spectrometry-based proteomics, including LiP-MS, single-cell proteomics, and proteome-wide PTM analyses related to neurodegenerative diseases.

The role involves data analysis, method development, and collaboration with the Center for Proteomics Discovery, with salary determined by NIH guidelines and a commitment to equal opportunity employment.

Qualifications

• Ph.D. or equivalent degree required.
• Experience in molecular biology or biochemistry preferred.
• Experience with mass spectrometry-based proteomics advantageous.

Responsibilities

• Conduct mass spectrometry-based proteomics projects (LiP-MS, single-cell proteomics).
• Analyze protein expression and post-translational modifications related to neurodegenerative diseases.
• Perform data analysis and statistics for project results.

Skills

Molecular biology

Biochemistry

Mass spectrometry

Statistics

Education

Ph.D. or equivalent degree

Tools

Orbitrap

timsTOF

Proteome Discoverer

Spectronaut

DIA-NN

Job description

Looking for apostdoctoral fellow who can do mass spectrometry-based proteomics:

The Na Lab at Johns Hopkins University School of Medicine is a Systems Biology lab that combines molecular biology, analytical chemistry, and computational biology with various "Omics" technologies, including genomics, proteomics, and metabolomics, to understand signaling pathways and to identify therapeutic targets and biomarkers in neurodegenerative diseases. Na lab developed in-situ cell-type-specific proteomics method using antibody-mediated biotinylation (iCAB) and is actively further advancing the iCAB method and applying it to various biomedical studies. Dr. Na is also the Director of the Center for Proteomics Discovery (CPD), and Na lab is closely working with CPD.

Job description:

The Na Lab is looking for a postdoctoral fellow who will work on various mass spectrometry-based proteomics projects such as the following:

• Structural proteomics using Limited Proteolysis Mass Spectrometry (LiP-MS), protein painting, and/or HDX
• Cell-type-specific, single-cell proteome analyses to uncover pathways involved in various diseases.
• Global and targeted identification and quantification of proteins and post-translational modifications involved in neurodegenerative diseases for the development of biomarkers and mechanistic-based studies.
• Mass spectrometry imaging for metabolites and lipids
• Data analysis and statistical analysis

Minimum Qualifications:

• Ph.D. or equivalent degree
• Experience in molecular biology or biochemistry

Preferred Qualifications:

• Ph.D. or equivalent degree
• Experience in operating Orbitrap or timsTOF instruments
• Experience in sample preparation for mass spectrometry-based proteomics
• Experience in the identification of post-translational modifications
• Good understanding of statistics
• Experience with Proteome Discoverer, Spectronaut, DIA-NN
• Knowledge of the maintenance of Orbitrap instruments
• Strong publication record

The annual salary will be determined based on NIH guidelines. A candidate who fits our requirements will be interviewed. All employment decisions shall be consistent with the principle of equal employment opportunity, and only job-related qualifications will be required.