NGS Scientist: Therapeutics Tech & Yeast Display

Kelly Science, Engineering, Technology & Telecom; Cambridge (MA); On-site; USD 75,000 - 110,000; Full time

Benefits offered by this job

Health insurance

401K

Paid holidays

PTO

Job summary

Kelly Science seeks a Scientist in Cambridge, MA to perform assay and workflow development for NGS, prepare and run samples on Illumina NextSeq and PacBio, and design analyses. The role involves independent data interpretation, technology development for therapeutics, and meticulous record-keeping in LIMS/ELN.

Responsibilities include cloning, plasmid prep, yeast culture and display, and preparing samples for FACS. Ideal candidates have 1+ year experience and strong collaborative skills.

Qualifications

• B.S. in molecular biology, biochemistry, biophysics, immunology, or related field with at least 1 year of relevant experience.
• Hands-on experience preparing and running samples on short-read or long-read NGS platforms.
• Proficiency in NGS library prep (short-read and/or long-read) and familiarity with NGS QC metrics and analysis tools.
• Hands-on experience in molecular biology techniques, including recombinant DNA cloning, sequence analysis, plasmid amplification, PCR, and gel purification.
• Skilled in yeast cell culturing, colony picking, and working with yeast display systems.
• Understanding of antibody structure and function.
• Communication and collaboration across diverse scientific teams.
• Industry experience and display-based experience (yeast, mammalian, or phage) preferred.
• Experience operating Illumina NextSeq and/or PacBio sequencing instruments (preferred).
• Attention to detail and regulatory-compliant record-keeping.

Responsibilities

• Performing assay and workflow development experiments for Next-Generation Sequencing (NGS) workflows.
• Preparing and running NGS samples on Illumina NextSeq and PacBio platforms.
• Independently designing, analyzing, and interpreting experimental results and technical reports.
• Participating in technology development efforts to drive long-term therapeutics discovery and validate technologies for portfolio readiness.
• Maintaining detailed and accurate records in LIMS and ELN, ensuring compliance with protocols and regulatory requirements.
• Cloning, transforming, and preparing plasmids as part of ongoing molecular biology workflows.
• Culturing yeast, picking colonies, and expressing proteins in yeast display systems.
• Preparing yeast cultures for FACS sorting and extracting DNA from selected populations.
• Registering DNA and protein sequences into the portal system for project banking.

Skills

NGS workflow development

DNA cloning and plasmid prep

Yeast culture & display

Data analysis & reporting

Education

Bachelor’s degree in molecular biology/biochemistry/biophysics/immunology or related field

Tools

Illumina NextSeq

PacBio

LIMS

Electronic Lab Notebook (ELN)

Job description

Kelly Science seeks a Scientist in Cambridge, MA to perform assay and workflow development for NGS, prepare and run samples on Illumina NextSeq and PacBio, and design analyses. The role involves independent data interpretation, technology development for therapeutics, and meticulous record-keeping in LIMS/ELN.

Responsibilities include cloning, plasmid prep, yeast culture and display, and preparing samples for FACS. Ideal candidates have 1+ year experience and strong collaborative skills.