Molecular & Cell Biology Senior Research Scientist

Vertex Pharmaceuticals LLC; Boston (MA); On-site; USD 122,000 - 182,000; Full time

Benefits offered by this job

Total rewards benefits

Paid time off

Educational assistance

Commuting subsidy

401(k)

Job summary

Vertex Pharmaceuticals LLC in Boston seeks a Senior Research Scientist, Molecular & Cell Biology to join the Human Cell Models group. You will develop and apply physiologically relevant neuronal models using iPSC and primary cells to advance disease biology and drug discovery.

The role emphasizes iPSC-derived neuronal models (dopaminergic, cortical neurons), genome editing (CRISPR/Cas9) and scalable assays, with collaboration across multidisciplinary teams to translate data into program

Qualifications

• PhD in a relevant life-science field with hands-on iPSC-derived neuronal model experience.
• Experience differentiating neuronal progenitors, cortical and dopaminergic neurons.
• Proven ability to establish reproducible iPSC-derived models and assays.

Responsibilities

• Design, execute, analyze, and communicate cellular/molecular studies for disease modeling.
• Develop neuronal models using iPSCs and primary cells, and apply scalable assays.
• Apply genome-editing and gene-modulation techniques to support objectives.
• Operate automated platforms and collaborate with cross-functional teams.

Skills

iPSC differentiation

neuronal model development

CRISPR/Cas9

flow cytometry

data analysis

cell culture

3D culture systems

bioreactors

imaging

Education

PhD in Stem Cell Biology, Developmental Biology, Neuroscience, Cell Biology, Molecular Biology, Genetics, Biomedical Sciences, or related

Tools

Echo

Hamilton

qPCR/RT-qPCR

Western blot

immunocytochemistry

immunofluorescence

Job description

Job DescriptionRole SummaryVertex is seeking a highly motivated Senior Research Scientist, Molecular & Cell Biology to join the Human Cell Models group in Boston Research. In this role, you will develop, characterize, and apply physiologically relevant human cellular models using stem cells and primary cells to deepen understanding of disease biology, identify therapeutic opportunities, and support the advancement of novel drug discovery programs.The successful candidate will bring deep expertise in disease modeling, with particular strength in neuronal model development using induced pluripotent stem cells (iPSC) and/or primary cells. This individual will have demonstrated experience developing neuronal models (i.e. dopaminergic neurons, cortical neurons, neuronal progenitors) using iPSCs including initial optimization and characterization through robust execution and application. The successful candidate will collaborate closely with multidisciplinary partners in early research, screening, pharmacology, chemistry, and computational genomics groups to generate high-quality data and translate scientific findings into actionable program decisions.Key Duties and Responsibilities:Design, execute, analyze, and communicate rigorous cellular and molecular biology studies supporting predictive disease modeling and drug discovery.Develop, optimize, characterize, and apply human neuronal models using primary cells, immortalized cell lines, iPSC-derived systems, and 3D culture approaches, as appropriate.Lead end-to-end iPSC model development, including reprogramming, cell-line maintenance, neuronal differentiation, quality assessment, assay readiness, and reproducible implementation.Apply molecular, cellular, biochemical, and functional assays to investigate disease mechanisms, validate targets, and assess therapeutic hypotheses.Develop scalable, quantitative cell-based assays for mechanistic studies, screening, pharmacology, and target-validation workflows.Use genome-editing and gene-modulation approaches—including CRISPR/Cas9, RNA interference, and overexpression—to support experimental objectives.Support stem cell expansion and differentiation through bioreactors, high-throughput culture systems, and optimized laboratory automation workflows.Operate, troubleshoot, and improve automated laboratory platforms, including Echo liquid handlers, Hamilton systems, plate washers, and related instrumentation.Interpret complex experimental data, recommend next-step studies, and integrate findings with genomic, transcriptomic, proteomic, imaging, and computational datasets in partnership with cross-functional teams.Advance scientific strategy and laboratory innovation through AI-enabled tools, emerging cell-modeling technologies, high-quality documentation, mentorship, and adherence to safety, quality, and data-integrity standards.Knowledge and Skills:Deep expertise in ESC and/or iPSC reprogramming, maintenance, expansion, characterization, and differentiation into neuronal lineages.Proven ability to independently establish, optimize, and implement reproducible iPSC-derived cell models and functional cell-based assays.Experience differentiating neuronal progenitors, cortical neurons, dopaminergic neurons, microglia, astrocytes, oligodendrocytes, and related cell types using chemical and/or transcription factor-based approaches.Strong hands-on capability in human primary cell culture, scalable stem-cell culture platforms, bioreactors, and cell-culture scale-up workflows.Experience operating, troubleshooting, and optimizing laboratory automation, including Echo liquid handlers, Hamilton platforms, plate washers, and related systems.Proficiency in molecular and cellular biology techniques, including flow cytometry and FlowJo, PCR/qPCR/RT-qPCR, Western blotting, ELISA, immunofluorescence, immunocytochemistry, and microscopy.Experience with CRISPR/Cas9, RNA interference, gene-expression modulation, plasmid design, transfection, transduction, and viral-vector approaches.Strong experimental design, statistical analysis, data interpretation, ELN documentation, and scientific data-management skills.Demonstrated scientific judgment, critical thinking, troubleshooting ability, and capacity to deliver high-quality, reproducible research results.Effective written, verbal, and presentation skills, with the ability to communicate findings to technical and nontechnical stakeholders.Collaborative, team-oriented mindset with the ability to manage multiple priorities in a fast-paced, cross-functional environment.Preferred: experience with 3D culture systems, organoids, co-cultures, microphysiological systems, high-content imaging, phenotypic screening, functional genomics, multi-omic data integration, and data-analysis tools such as GraphPad Prism, R, or Python.Education and Experience:PhD in Stem Cell Biology, Developmental Biology, Neuroscience, Cell Biology, Molecular Biology, Genetics, Biomedical Sciences, or a related scientific discipline.At least four years of relevant postdoctoral and/or industry experience.Significant hands-on experience developing and applying iPSC-derived neuronal models in a research setting.Experience supporting therapeutic discovery programs within a biotechnology, pharmaceutical, academic, or similarly rigorous research environment is preferred.At Vertex, we embrace continuous learning and encourage employees to stay informed on advances in AI, technology, automation, and industry trends. We seek talent who bring an external perspective, demonstrate curiosity and sound judgment, and thoughtfully adopt new capabilities that improve decision-making, drive innovation, increase efficiency, and help deliver better outcomes for patients.Pay Range:$121,600 - $182,400Disclosure Statement:The range provided is based on what we believe is a reasonable estimate for the base salary pay range for this job at the time of posting. This role is eligible for an annual bonus and annual equity awards. Some roles may also be eligible for overtime pay, in accordance with federal and state requirements. Actual base salary pay will be based on a number of factors, including skills, competencies, experience, and other job-related factors permitted by law.At Vertex, our Total Rewards offerings also include inclusive market-leading benefits to meet our employees wherever they are in their career, financial, family and wellbeing journey while providing flexibility and resources to support their growth and aspirations. From medical, dental and vision benefits to generous paid time off (including a week-long company shutdown in the Summer and the Winter), educational assistance programs including student loan repayment, a generous commuting subsidy, matching charitable donations, 401(k) and so much more.Flex Designation:On-Site DesignatedFlex Eligibility Status:In this On-Site designated role, you will work five days per week on-site with ad hoc flexibility.Note: The Flex status for this position is subject to Vertex’s Policy on Flex @ Vertex Program and may be changed at any time.#LI-OnsiteCompany InformationVertex is a global biotechnology company that invests in scientific innovation.Vertex is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, sex, gender identity or expression, age, religion, national origin, ancestry, ethnicity, disability, veteran status, genetic information, sexual orientation, marital status, or any characteristic protected under applicable law. Vertex is an E-Verify Employer in the United States. Vertex will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.Any applicant requiring an accommodation in connection with the hiring process and/or to perform the essential functions of the position for which the applicant has applied should make a request to the recruiter or hiring manager, or contact Talent Acquisition at [e-mail]