Erle Lab - Junior Specialist

University of California, San Francisco; San Francisco (CA); On-site; USD 48,280 - 65,320; Full time

Job summary

University of California, San Francisco in San Francisco, CA is recruiting a Junior Specialist to perform experiments exploring mechanisms of asthma, including mucus plugging and airway epithelial changes. The role uses CRISPR systems, primary human airway cells, and various assays under PI supervision.

The candidate will design and interpret experiments, help maintain lab supplies, and collaborate with lab and partner labs to advance related projects, with a focus on wet-lab CRISPR techniques

Qualifications

• One year of experience in a lab with at least two methods among cell culture, flow cytometry, molecular biology, tissue staining or microscopy.
• Computational experience with Python or R is a plus.

Responsibilities

• Design, conduct, and interpret experiments under supervision of the PI.
• Maintain lab supplies and equipment.
• Collaborate with PI's lab and others to support related projects.

Skills

Mammalian cell culture

Flow cytometry

Molecular biology

Microscopy

Python

Education

Bachelor's degree or 4 years experience

Job description

Position Description

The Erle Lab is now recruiting for a Junior Specialist. The junior specialist will perform experiments designed to help understand fundamental mechanisms underlying asthma. Changes in airway epithelial cells and airway mucus are important contributors to asthma. In severe asthma, mucus plugs obstruct the airway and we are conducting studies to understand the causes of mucus plugging. The laboratory uses primary cultures of human airway epithelial cells and tests the roles of specific genes using CRISPR-based and other approaches. Position duties include: 1) designing, conducting, and interpreting experiments under the supervision of the PI; 2) helping to maintain lab supplies and equipment; and 3) working with other members of the PI's lab and collaborating labs to support related projects. Methods employed include human primary cell culture, lentiviral and other CRISPR systems, flow cytometry, immunofluorescence, videomicroscopy, and immunoblotting.

Required Qualifications

• Must have (or be in the process of obtaining) a baccalaureate degree (or equivalent degree) or at least four years of research experience.

Preferred Qualifications

• One year of experience in a research laboratory involving two or more of the following methods: mammalian cell culture, flow cytometry, molecular biology (PCR, plasmid preparation), tissue staining/microscopy.
• Computational experience (e.g., experience with using Python or R).

Applicants must meet the required qualifications by time of hire.

Please apply here. Applicants’ materials must list current and/or pending qualifications upon submission.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $55,000-$58,600.

Document Requirements

• Curriculum Vitae – CV must clearly list current and/or pending qualifications (e.g., board eligibility/certification, medical licensure, etc.).
• Cover Letter (Optional)
• Statement of Research (Optional)
• Statement of Teaching (Optional)

A University employee must comply with all applicable University policies and/or collective bargaining agreements. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

UCSF is committed to welcoming and serving all people, honoring the dignity of every individual without preference or prejudice, in support of its public mission and in alignment with our PRIDE values and Principles of Community.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

• Misconduct means any violation of the policies or laws governing conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer.
• UC Sexual Violence and Sexual Harassment Policy
• UC Anti-Discrimination Policy
• APM - 035: Affiliation and Nondiscrimination in Employment

San Francisco, CA