Enzyme Engineering Postdoc: Cell-Free Systems

National Renewable Energy Laboratory; Golden (CO); On-site; USD 76,600 - 126,400; Full time

Job summary

National Renewable Energy Laboratory in Golden, Colorado, invites applications for a Postdoctoral Researcher in enzyme engineering for cell-free systems. The project aims to integrate cell-free workflows with genome engineering to accelerate biosystems design for carbon-negative biomanufacturing, developing optimized cell-free platforms for converting C1 substrates into biofuels and bioproducts.

The role encompasses identifying enzymes, building high-throughput assays, and collaborating with

Qualifications

• Recent PhD earned within the last three years.
• Experience cloning, expressing, and purifying proteins.
• Experience automating biochemical assays.
• Proven enzyme or protein engineering background.
• Ability to follow SOPs with attention to detail.
• Strong publication record.
• Excellent safety record.
• Initiative to set task direction.

Responsibilities

• Identify, express, purify, and assay enzymes from metabolic pathways.
• Develop high-throughput enzymatic assays to validate pathways.
• Collaborate with data scientists to design cell-free production pathways.
• Perform directed evolution and machine-learning-guided protein engineering.
• Conduct experiments safely with minimal supervision.
• Plan and execute research with multidisciplinary teams.
• Communicate results in writing and oral formats.
• Author peer-reviewed publications.
• Work in a large, multi-institutional team.

Skills

Cloning

Protein expression

Protein purification

Enzyme engineering

Automation of assays

Cell-free systems

Directed evolution

Scientific troubleshooting

Publications

Safety discipline

Education

PhD in relevant field

Tools

HPLC

GC-FID

GC-MS

LC-MS

Job description

National Renewable Energy Laboratory in Golden, Colorado, invites applications for a Postdoctoral Researcher in enzyme engineering for cell-free systems. The project aims to integrate cell-free workflows with genome engineering to accelerate biosystems design for carbon-negative biomanufacturing, developing optimized cell-free platforms for converting C1 substrates into biofuels and bioproducts.

The role encompasses identifying enzymes, building high-throughput assays, and collaborating with