Downstream Processing Intern: EVs & Viral Vector Purification

Sartorius North America; Marlborough (MA); On-site; USD 34,000 - 49,000; Full time

Benefits offered by this job

Personal and Professional Development

Making an Impact Right from the Start

Welcoming Culture

Intelligent Working Environment

Job summary

Sartorius North America invites applications for a Research Intern position onsite at our Marlborough, MA site. The PhD-level intern will support R&D activities in downstream processing of extracellular vesicles (EVs) and viral vectors, optimizing purification workflows using TFF and chromatography.

This role offers hands-on biologics processing experience and data-driven process development for therapeutic nanoparticles.

Qualifications

• Hands-on experience with downstream purification workflows (TFF) for nanoparticles or biologics.
• Knowledge of chromatography techniques such as SEC, IEX, or affinity purification.
• Familiarity with extracellular vesicles (EVs) and viral vectors (e.g., AAV, lentivirus).
• Comfortable working in a wet lab with complex biological samples.
• Strong documentation and batch-record practices are a plus.

Responsibilities

• Execute purification protocols for EVs and viral vectors using TFF systems and chromatography modalities.
• Assist in designing and optimizing downstream steps to improve yield, purity, and scalability.
• Operate and maintain TFF and chromatography systems (e.g., AKTA), including setup and cleaning.
• Prepare and process samples following SOPs; quantify particles using NTA, qPCR, protein assays, and RNA analysis.
• Monitor and document key parameters during TFF runs; maintain lab notebooks and process logs.
• Support integration of downstream data with analytical assays (e.g., flow cytometry, SEC‑MALS).
• Collaborate with upstream, analytical, and formulation teams to align on goals and knowledge transfer.
• Follow lab safety protocols and ensure compliance with quality standards.

Skills

TFF systems

Chromatography techniques

EVs purification

Analytical quantification

Lab documentation

Education

PhD candidate in Bioengineering or closely related discipline

Tools

AKTA

NTA

qPCR

HPLC

SEC‑MALS

Job description

Sartorius North America invites applications for a Research Intern position onsite at our Marlborough, MA site. The PhD-level intern will support R&D activities in downstream processing of extracellular vesicles (EVs) and viral vectors, optimizing purification workflows using TFF and chromatography.

This role offers hands-on biologics processing experience and data-driven process development for therapeutic nanoparticles.