Cell Assay Senior Scientist - Blue Sky Kinases

St Jude Children's Research Hospital; Memphis (TN); On-site; USD 104,000 - 186,000; Full time

Job summary

St. Jude Children's Research Hospital in Memphis, TN seeks an experienced Senior Scientist to join the Blue Sky Initiative on kinases. You will leverage molecular biology, biochemistry, and cell culture to translate kinase structure into cellular function.

The role involves developing and optimizing cell-based assays across multiple platforms, collaborating across structural biology, proteomics, and data science, and contributing to high-impact pediatric research.

Qualifications

• Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.
• Expertise in culturing a variety of cell types including cancer lines, primary cells, and iPSC-derived cells.
• Experience with multiple methods for exogenous protein expression.
• Proficiency in presenting experimental design and results using PowerPoint or similar.
• Experience with mouse models is preferred but not essential.

Responsibilities

• Research kinase-mediated signaling pathways and cellular outcomes for assigned projects.
• Identify cellular assays and cells for structure-guided analysis of kinase signaling.
• Develop strategies for cell-based assays, including protein phosphorylation, interaction, localization, and qRT-PCR.
• Develop strategies for phenotypic assays such as viability, apoptosis, autophagy, migration, or differentiation.
• Perform optimization and troubleshooting of cell-based assays.
• Perform statistical analysis using graphing software or R scripts.
• Interpret and communicate results within the team and with collaborators.

Skills

Kinase signaling analysis

Cell culture expertise

Exogenous protein expression

PowerPoint presentation

Mouse models experience

Education

Bachelor's degree in relevant scientific area

Tools

Cloning/PCR/qRT-PCR

Transfection/Transduction

Immunoassays (ELISA/AlphaLISA)

Immunoprecipitation & Western blot

Flow cytometry/FACS

Microscopy & imaging

Job description

We are looking for an experienced cell biologist to participate in our exciting studies of the human protein kinome.

This Senior Scientist position is part of the Blue Sky Initiative "Seeing the Invisible in Protein Kinases" at St. Jude Children's Research Hospital in Memphis, TN.

As a member of the Cell-Based Assays team, the candidate will use their molecular biology, biochemistry, and cell culture skills to perform research that translates novel insights regarding kinase structure and regulation in vitro into functional effects in cells. Additionally, the candidate will assist in performing cell-based analysis in high-, medium-, and low-throughput platforms of candidate inhibitors of kinases.

The work is diverse providing an opportunity to study multiple kinases, perform appropriate background research, optimize cell-based assays of various types using many different cell lines or primary cells. Not only are the cell-based studies used to validate the structural findings, but they are also used to discover new kinase-mediated biology or new mechanisms of kinase regulation.

The Blue Sky Initiative "Seeing the Invisible in Protein Kinases" is an inter-disciplinary project in the Department of Structural Biology and is supported by US $50 million in institutional funding. This large-scale project leverages ultra-high field nuclear magnetic resonance (NMR), complemented with cryoEM, X-ray crystallography, data-science and computational approaches, to characterize the conformational landscape of the human kinome. Knowledge of the conformations of kinases is then used to discover new biology regarding kinase function and regulation in cells, understand the structural and functional consequences of disease-associated mutations, and identify novel therapeutic opportunities.

Responsibilities

• Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects
• Identify appropriate cellular assays and cells to perform structure-guided analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function
• Develop strategies and plans for implementing cell-based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance
• Develop strategies and plans for implementing cell-based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation
• Perform optimization and troubleshooting of cell-based assays
• Perform statistical analysis using graphing software or R scripts
• Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects

Experience and Expertise

• Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.
• Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.
• Expertise in multiple methods for exogenous protein expression.
• Proficiency in presentation of experimental design and results using PowerPoint or similar software.
• Experience with mouse models, preferred but not essential.

Technical Capabilities

• Cloning, PCR, and qRT-PCR
• Sterile technique
• Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation
• Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery

• Immunoprecipitation and Western blotting
• Protein-protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays
• Flow cytometry and fluorescence-activated cell sorting (FACS)
• Reporter gene assays
• Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony-forming, autophagy, endocytosis, secretion, or differentiation assays
• Microscopy, such as immunofluorescence, live-cell imaging, subcellular localization, colocalization analysis
• Cellular fractionation

Competencies

• Creative and results-driven with strong critical thinking and the ability to learn quickly
• Hardworking, well-organized, flexible, and capable of multitasking efficiently in a fast-paced environment
• Collaborative and efficient member of teams with diverse backgrounds and opinions
• Ability to prioritize work and complete assignments promptly

Minimum Education and Experience

• Bachelor's degree in relevant scientific area is required.

Minimum Experience

• Five (5) years of relevant work experience is required with a PhD.
• Eight (8) years of relevant experience is required with a Master's Degree.
• Ten (10) years of relevant work experience is required with a Bachelor's Degree.

The World's Brightest Minds Always Innovate

At St. Jude Children's Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That's why we provide

• World-class facilities
• State-of-the-art technologies
• Extraordinary support
• A collaborative, bench-to-bedside environment

You will see, firsthand, how your science translates into survival for the children that we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.

Better at Work. Better at Life.

When you work at St. Jude, you'll join a highly collaborative work culture that inspires you every day to be your best. With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world-class institution. This includes work-life balance with generous paid time-off and on-campus conveniences that make life a little easier. Join us and you'll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a "Best Place to Work."

Have More in Memphis

Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people, Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can " Have More in Memphis ."

Compensation

In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $104,000 - $186,160 per year for the role of Cell Assay Senior Scientist - Blue Sky Kinases.

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