Avian Viral Vector Scientist - Vector Engineering

Neion Bio, Inc.; Northern, New York (KY, NY); Hybrid; USD 120,000 - 150,000; Full time

Benefits offered by this job

Health insurance

Dental insurance

Vision insurance

401(k)

Equity compensation

Job summary

Neion Bio is pioneering cost-effective, scalable viral vector production using chicken egg platforms. We seek a talented virologist or molecular biologist at the scientist level to engineer, produce and purify viral vectors, including lentivirus, adenovirus and AAV, and to deepen understanding of receptor biology in avian systems.

This is a hands-on role. This position involves cloning large viral genomes, transfections, vector design, purification, data analysis, and rigorous GLP practices,

Qualifications

• PhD or BA with 7+ years of experience/MS with 5+ years in biological sciences, bioengineering, molecular biology, genetics, or related field.
• Experience with cloning large libraries or large viral genomes.
• Experience with transfection and transduction of difficult target cells/tissues.
• Experience producing, purifying, characterizing, validating and testing viral vectors including adenovirus, lentivirus, AAV.
• Deep understanding of cellular biology, receptors for viral entry and trafficking, mechanisms involved in viral transduction, and immunology.
• Experience with ultracentrifugation and resin/column based viral purification methods.
• Genome engineering, cloning, DNA amplification, PCR, NGS, transfections, and other molecular biology skills.
• Strong aseptic techniques and GLP procedures.
• Exceptional organizational skills with the ability to effectively manage time and priorities.
• Strong written and oral communication skills, as well as creative problem-solving skills.
• Curious, eager, and hardworking

Responsibilities

• Perform cloning of large viral genomes, with focus on capsid modification by design or directed evolution.
• Perform transfections on primary cells, immortalized cells, and animal tissues.
• Design, manufacture, purify, characterize, validate and test a range of viral vectors at small and large scale.
• Design experiments, generate and analyze datasets to adapt vector strategies for avian systems.
• Assess efficiency of workflows through cellular assays and NGS sequencing approaches.
• Extract DNA, RNA, protein from animal tissue and run PCR/qPCR/RT-PCR/ELISA to quantify viral transgene and transcription, protein.
• Conduct experiments and work on research problems.
• Maintain excellent electronic laboratory records.
• Attend lab meetings and prepare reports/presentations on project progress.

Skills

Strong organizational skills

Strong written and oral communication

Creative problem-solving

Curious, eager, hardworking

Aseptic techniques

GLP procedures

Education

PhD or BA with 7+ years or MS with 5+ years in biological sciences

Tools

Cloning large libraries / viral genomes

Transfection and transduction of difficult cells/tissues

Ultracentrifugation purification methods

DNA amplification and PCR/NGS workflows

Job description

Neion Bio is pioneering cost-effective, scalable viral vector production using chicken egg platforms. We seek a talented virologist or molecular biologist at the scientist level to engineer, produce and purify viral vectors, including lentivirus, adenovirus and AAV, and to deepen understanding of receptor biology in avian systems.

This is a hands-on role. This position involves cloning large viral genomes, transfections, vector design, purification, data analysis, and rigorous GLP practices,