Asst Research Scientist/Engin - Li Lab

The University of Iowa; Iowa City (IA); Vor Ort; USD 51.000 - 62.000; Vollzeit

Zusammenfassung

The University of Iowa's Department of Pathology seeks an Assistant Research Scientist to study how A-FABP and E-FABP regulate cell metabolism and signaling. This 100% time position involves designing and conducting projects, collecting high-quality data, and training lab personnel.

Responsibilities include cell culture, PCR, Western blotting, ELISA, flow cytometry, mouse colony maintenance, and collaboration with students and technicians. A PhD in Immunology or related field is required.

Qualifikationen

• Ph.D. in Immunology or related field with a strong publication record.
• Ability to independently design, optimize and perform experiments.
• Experience presenting at professional meetings and collaborating across teams.

Aufgaben

• Cell culture of lines and primary immune cells; establish stable lines or CRISPR knockouts.
• Prepare single-cell suspensions from tissues and perform analyses.
• Perform real-time PCR, Western blotting, RNAi; immunoassays (ELISA, immunofluorescence).
• Maintain mouse colonies and genotype with assistance; assist technicians.
• Prepare, run and analyze multi-color flow cytometry; troubleshoot procedures.

Kenntnisse

Independent experimentation design

Communication skills

Teamwork

CRISPR technique

Ausbildung

PhD in Immunology or related field

Tools

Real-time PCR

Western blotting

ELISA

Immunofluorescence

Flow cytometry

Genotyping

Jobbeschreibung

The Department of Pathology is seeking an Assistant Research Scientist to investigate the mechanisms of how A-FABP and E-FABP regulate cell metabolism and intracellular signaling pathways in cells from various types of disease models like obesity, cancer and psoriasis. Tasks include the design and performance assigned research project and obtain high-quality data in each individual study. Tasks also include management of laboratory protocols, training and collaboration of laboratory personnel, including students, post-doctoral fellows and technicians. This position is 100% time.

Duties to include:

· Cell culture for cell lines, primary immune cells like BMDC, BMDM, establishing cell lines of stable gene expression or knockout using CRISPR technique;

· Preparation of single cell suspension from tissues.

· Highly skilled on real-time PCR, western blotting, RNAi;

· Perform antibody generation, isolation and purification;

· Perform ELISA from serum and supernatant;

· Perform Immunofluorescence staining using cells and tissues;

· Maintenance of mouse colonies and genotyping with assistant of technician;

· Prepare, perform and analyze samples using multi-color flow cytometry;

· Troubleshoots with issues for experimental procedures and updates as needed.

The Department of Pathology is part of UI Health Care, a comprehensive health sciences center that serves Iowa and the region, as well as patients referred from the United States and overseas. The Clinical Laboratories are well recognized for providing outstanding and comprehensive care to the patients we serve. The breadth and depth of services provided are amongst the best in the country. The educational programs that the department participates in includes medical students, clinical laboratory scientists as well as biomedical scientists.To learn more: https://pathology.medicine.uiowa.edu/about-us

Required Qualifications:

· Ph.D. in Immunology or related field

· At least 1 year of postdoctoral experience in Immunology or cancer-related field.

· Demonstration of strong ability to independently design, optimize and perform experiments, establishing experimental protocols to solve scientific questions.

· Evidence with a record of publications in peer-reviewed journals.

· Experience of establishing a national presence with talks, abstract presentations and/or participation at professional meetings.

· Excellent communication skills and integrating positive effort to teamwork.

Desirable Qualifications:

· At least 3 years of postdoctoral experience in Immunology or cancer-related field.

· Prior experience with developing mouse models on high fat diet/tumor implantation, and compliance with IACUC regulations is highly desired.

· Highly skilled in multi-color Flow cytometry to identify immune cells (T cell, macrophages) is highly desired.

· Standard molecular biological and immunological techniques is highly desired.

Successful candidates will be subject to a criminal background check and credential/education verification. Up to 5 professional references will be requested at a later step in the recruitment process.

• Classification Title: Asst Research Scientist/Engin
• Appointment Type: Professional and Scientific
• Schedule: Full-time
• Work Modality Options: On Campus

Compensation

• Starting Salary Minimum: $56,697
• Starting Salary Maximum: Commensurate

Contact Information

• Contact Email: [e-mail]

Equal opportunity employer

The University of Iowa is an equal opportunity employer. All qualified applicants are encouraged to apply and will receive consideration for employment free from discrimination on the basis of race, creed, color, religion, national origin, age, sex, pregnancy (including childbirth and related conditions), disability, genetic information, status as a U.S. veteran, service in the U.S. military, sexual orientation, or associational preferences.

Persons with disabilities who need assistance or accommodations with the application or interview process may contact University Human Resources/Faculty and Staff Disability Services, [phone] or [e-mail] . For jobs in UI Health care, please contact UI Health care Leave & Disability Administration at 319-356-7543 .