Associate Researcher I (Dr Dubois' Lab) - Cell Developmental & Regenerative Biology

Mount Sinai; EE. UU.; Presencial; USD 60.000 - 80.000; Jornada completa

Descripción de la vacante

Mount Sinai's Dubois Laboratory seeks an Associate Researcher I to join our cardiovascular biology team. You will perform mammalian cell culture, manage mouse colonies, and apply molecular biology techniques to study heart development and disease.

The role offers hands-on training, collaborative projects, and opportunities to contribute to publications. Ideal candidates have a Bachelor's or Master's in a science field, with interest in developmental or cardiac biology, and familiarity with PCR,

Formación

• Bachelor's or Master's degree in science or related field required.
• Hands-on mammalian tissue culture experience preferred.
• Familiar with basic molecular biology methods (PCR, gel electrophoresis, immunostaining).
• Experience with mouse husbandry, genotyping, and colony management is a plus.

Responsabilidades

• Perform mammalian cell culture, including pluripotent stem cell lines and cardiac cell types.
• Assist with mouse colony management, husbandry, and genotyping.
• Conduct PCR, cloning, Western blotting, immunofluorescence, and qRT-PCR.
• Assist with tissue dissection, histology, and immunostaining on cardiac tissue.
• Maintain detailed lab records and contribute to figures and publications.
• Participate in lab meetings and support routine operations.

Conocimientos

Mammalian cell culture

Mouse colony management

PCR

Cloning

Western blotting

Immunofluorescence

qRT-PCR

Histology

Immunostaining

Lab documentation

Educación

Bachelor's or Master's in science or related field

Herramientas

PCR

Gel electrophoresis

Western blot

Immunostaining

Confocal microscopy

Flow cytometry

Descripción del empleo

The Dubois Laboratory at the Icahn School of Medicine at Mount Sinai investigates the fundamental mechanisms of cardiac development, with a focus on understanding how the heart is built at the cellular and molecular level. Our research spans questions in cardiac progenitor specification, ventricular chamber morphogenesis, cardiomyocyte maturation, and congenital heart disease, using a combination of mouse genetics, stem cell models, and genomic and imaging technologies. We are a collaborative and collegial team passionate about uncovering the fundamental mechanisms that drive the formation and function of the heart.

https://labs.icahn.mssm.edu/duboislab/

The Dubois Laboratory is seeking a motivated and enthusiastic Associate Researcher I to join our team immediately. This is an excellent opportunity for an individual who is passionate about cardiovascular biology and eager to contribute to research in a dynamic and supportive environment. The successful candidate will work on a range of projects investigating fundamental questions in cardiac development and disease, and will have the opportunity to develop a broad and versatile technical skill set. We are looking for someone who thrives both working independently and contributing as part of a collaborative team.

Responsibilities

• Perform mammalian cell culture, including maintenance of pluripotent stem cell lines and differentiated cardiac cell types
• Assist with mouse colony management, husbandry, and genotyping
• Conduct standard molecular biology techniques including PCR, cloning, Western blotting, immunofluorescence, and qRT-PCR
• Assist with tissue dissection, histology, and immunostaining on embryonic and adult cardiac tissue
• Maintain detailed and organized laboratory records and experimental documentation
• Contribute to the preparation of figures, protocols, and other materials supporting publications and grant applications
• Participate actively in laboratory meetings and contribute to a collaborative team environment
• Assist with general laboratory operations including ordering supplies and maintaining equipment
• Performs other related duties

Requirements

• Bachelors or Masters degree in science or related field required
• No experience required. One year of research experience preferred.
• Highly Preferred: Hands-on experience with mammalian tissue culture techniques
• Familiarity with basic cell and molecular biology methods (PCR, gel electrophoresis, Western blotting, immunostaining)
• Experience with mouse husbandry, colony management, and genotyping
• Hands-on experience with mammalian tissue culture techniques
• Prior exposure to developmental biology and/or cardiac biology
• Experience with confocal microscopy or other imaging modalities
• Familiarity with flow cytometry/FACS
• Experience in a research laboratory setting beyond coursework