Advanced Research Assistant, Immune Cell Genomics

Cincinnati Children's Office of Academic Affairs and Career Development; Cincinnati (OH); On-site; USD 57,000 - 72,000; Full time

Job summary

Cincinnati Children’s Hospital Medical Center in Cincinnati, Ohio seeks a Research Assistant IV to conduct independent and directed studies on immune cells and nuclei preparation for multiome analysis. You will design experiments, reproduce and optimize protocols, and train junior staff in molecular biology techniques.

The role involves Western blot, flow cytometry, DNA/RNA/protein extraction, and library preparation for ATAC-seq/ChIP-seq, with emphasis on data analysis and collaboration across

Qualifications

• Bachelor's degree or higher in chemistry, biology, molecular biology, biotechnology, or biochemistry.
• 1 year of experience in conducting independent and directed scientific studies and research in a lab setting.
• 1 year of experience in isolating and stimulating immune cells in a biosafety level 2 laboratory and optimizing subsequent molecular protocols to be used to assess these cells.
• 1 year of experience in working independently and as part of a team in an academic research lab.
• 1 year of experience in isolating inflamed blood cells from small amounts (less than 3 mL) of peripheral blood.
• 1 year of experience in isolating RNA, DNA, chromatin, nuclear lysates, and cytoplasmic lysates from cells with subsequent quality assessment of isolated materials.
• 1 year of experience in performing Western blot analysis with proficiency in Empiria Studio.
• 1 year of experience in performing flow cytometry to measure expression of proteins on a single cell level using antibody staining for extracellular and intracellular epitopes with proficiency in DB FACSDiva and FlowJo for quantification and analysis.
• 1 year of experience in development of research protocols.
• 1 year of experience in optimizing biochemical protocols with primary cells derived from inflamed tissues.
• 2 years of experience in providing Material Safety Data Sheet (MSDS) training.
• 2 years of experience in handling disposal of chemicals.
• 1 year of experience collaborating and coordinating with a physician around experimental logistics of obtaining research samples from a clinical encounter.

Responsibilities

• Conduct independent and directed scientific studies in support of PI research.
• Design and perform experiments; record data and verify published procedures.
• Prepare libraries and perform assays for ATAC-seq and ChIP-seq.
• Isolate, stimulate, and analyze immune cells; optimize molecular protocols.
• Maintain detailed records and quality checks on lab work and materials.
• Train postdocs, graduate students, and junior staff in techniques.

Skills

Independent research

Team collaboration

Education

Bachelor's degree in chemistry, biology, molecular biology, biotechnology, or biochemistry

Job description

Cincinnati Children’s Hospital Medical Center in Cincinnati, Ohio seeks a Research Assistant IV to conduct independent and directed studies on immune cells and nuclei preparation for multiome analysis. You will design experiments, reproduce and optimize protocols, and train junior staff in molecular biology techniques.

The role involves Western blot, flow cytometry, DNA/RNA/protein extraction, and library preparation for ATAC-seq/ChIP-seq, with emphasis on data analysis and collaboration across