Research Fellow (Chemistry)

NATIONAL UNIVERSITY OF SINGAPORE; Singapore; On-site; SGD 70,000 - 110,000; Full time

Job summary

National University of Singapore seeks a highly skilled researcher to advance the study of RNA modifications and RNA binding proteins within the cfRNA-mod project. You will establish model systems, perform biochemistry and cell biology experiments, and contribute to bioinformatics analyses for associated omics data.

You will lead a small group of PhD researchers, assist with grant applications, and provide mentorship, reporting to the principal investigator.

Qualifications

• PhD in Biochemistry and Molecular Biology or related discipline.
• Extensive bench experience in biochemistry, molecular biology, chemical biology, genetics and cell biology.
• Proven track record with peer-reviewed publications (first-author preferred).

Responsibilities

• Establish model system to study context-dependent crosstalk between epitranscriptome and epigenome.
• Perform biochemistry, cell biology experiments and bioinformatics for omics data.
• Assist in grant applications and support junior PhD students.
• Lead a small group of PhD students on RNA modification and RBPs in development.
• Report progress to PI.

Skills

Biochemistry techniques

Molecular biology

Cell biology

CRISPR-Cas9 genome editing

NGS library preparation

Flow cytometry

Immunoblotting / Western

Education

PhD in Biochemistry or Molecular Biology

Tools

NGS data analysis

CRISPR-Cas9 tools

Western blot

Flow cytometry

Job description

We regret that only shortlisted candidates will be notified.

Job Description

The successful candidate will work with Asst Prof WEI Jiangbo on physiological functions of RNA modifications and RNA binding proteins under a project on "Circulating Cell-Free RNA modification (cfRNA-mod) for MASH Diagnosis/Monitoring".

The main responsibilities of the position include:

1. Establish the model system to study context-dependent crosstalk between epitranscriptome and epigenome in the lab.
2. Carry out biochemistry and cell biology-related experiments and bioinformatics work for associated omics.
3. Facilitate grant application.
4. Aid and support to junior PhD students in their research projects.
5. Lead a small group of PhD working on the physiological roles of RNA modifications and RBP in development.
6. Report duty to PI.

Qualifications / Discipline:

• The candidate should hold a PhD degree or havesubmitted PhD thesis, in Biochemistry and Molecular Biology, or related disciplines.

Skills:

• Proficient in a wide range of bench techniques relevant to biochemistry, molecular biology, chemical biology, genetics, and cell biology.
• Skills include, but are not limited to: chemical synthesis, nucleic acid extraction and purification, molecular cloning, plasmid preparation, PCR and RT-qPCR, site-directed mutagenesis, restriction enzyme digestion, ligation, gel electrophoresis (agarose and SDS-PAGE), immunoblotting (Western blot), immunoprecipitation, ELISA, cell culture (bacterial, yeast, and mammalian), cell transfection and transduction, CRISPR-Cas9 genome editing, Sanger and next-generation sequencing (NGS) library preparation, immunofluorescence and confocal microscopy, flow cytometry, protein expression and purification, enzyme assays, and spectrophotometric analysis.
• Has extensive experience in model system that suitable to study the role of RNA modifications and RBPs in development.

Experience:

• Have published (or at least submitted) peer-reviewed papers as the first author in high-impact journals.