MSCA-DN PhD position in Molecular Pharmacology (Amsterdam, NL)

AcademicTransfer; Netherlands; On-site; EUR 36,000 - 45,000; Full time

Job summary

Vrije Universiteit Amsterdam is seeking a highly motivated candidate for a POLARIS doctoral network project in medicinal chemistry and pharmacology. You will study an orphan GPCR as a potential drug target for megalencephalic leukoencephalopathy with subcortical cysts, using cellular assays and high-throughput screening to identify modulators.

You will perform secondments, training, and collaborations across EU partners, contributing to assay development and lead optimization within an

Qualifications

• Master's degree in pharmaceutical sciences, molecular neuroscience, biomedical sciences, medicinal chemistry or a comparable life sciences discipline.
• Experience in molecular pharmacology and/or receptor biochemistry with cell culture or cell-based signaling assays.
• Strong interest in GPCR biology and in developing cell-based assays with high-throughput readers and robotics.
• Experience with drug discovery is an advantage.
• Experience with molecular biology techniques and fluorescence microscopy is an advantage.
• Accurate, reproducible approach with strong analytical, communication and organizational skills; ability to collaborate across research environments.
• Fluency in spoken and written English is essential.

Responsibilities

• Develop cell-based bioluminescent and fluorescent biosensor assays to detect GPCR signalling events in different cellular models.
• Investigate receptor activation and signalling mechanisms.
• Develop and apply high-throughput screening assays to identify potential drug‑like modulators.
• Use FDA‑approved drug library repurposing and fragment‑based drug discovery to identify and characterize promising compounds.
• Contribute to the development of a functional GPCR assay pipeline and the evaluation of lead molecules in collaboration with POLARIS partners.
• Planned secondments provide hands‑on experience in in vitro assay validation and in vivo characterisation of lead molecules.

Skills

Molecular pharmacology

Receptor biochemistry

Cell-based assays

Fluorescence biosensors

GPCR biology

Drug discovery

Molecular biology

English fluency

Education

Master's degree in pharmaceutical sciences, molecular neuroscience, biomedical sciences, medicinal chemistry or a comparable life sciences discipline

Tools

High-throughput screening

Job description

Pharmacological sciences » Clinical pharmacology

Organisation/Company Amsterdam UMC Research Field Medical sciences » Medicine Pharmacological sciences » Clinical pharmacology Researcher Profile First Stage Researcher (R1) Application Deadline 5 Oct 2026 - 21:59 (UTC) Country Netherlands Type of Contract Permanent Job Status Not Applicable Hours Per Week 36.0 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

POLARIS doctoral network

POLARIS is a European Marie Skłodowska‑Curie Doctoral Network focused on accelerating therapy development for leukodystrophies: rare genetic disorders affecting the brain's white matter. The network aims to strengthen the full translational pathway, from understanding disease mechanisms and developing disease models to preclinical therapy testing, clinical readiness and patient‑informed treatment priorities.

To achieve this, POLARIS brings together expertise in leukodystrophy biology, advanced disease models, therapeutic innovation, biomarker development, clinical translation, patient engagement, and ethical, regulatory and health‑economic aspects of therapy development. The network will train a cohort of doctoral candidates in an international and interdisciplinary environment, combining academic research, industry‑based preclinical development, secondments, network‑wide training and transferable‑skills development.

PhD project

The project is based within the Medicinal Chemistry research group at Vrije Universiteit Amsterdam. You will investigate the molecular pharmacology of an orphan G protein‑coupled receptor (GPCR) as a potential drug target for megalencephalic leukoencephalopathy with subcortical cysts (MLC), a rare genetic brain disease that affects mechanisms regulating brain volume.

MLC currently has no available drug treatment. The orphan GPCR was recently identified as a potential drug target in a subgroup of people with MLC by researchers in Pediatric Neurology at Amsterdam UMC. GPCRs are cell‑surface proteins that respond to external stimuli and are widely investigated as drug targets.

The aim of the project is to understand how the orphan receptor is activated and signals, develop high‑throughput screening assays and identify drug‑like modulators. You will use FDA‑approved drug library repurposing and fragment‑based drug discovery (FBDD) to search for potential modulators.

As part of the POLARIS doctoral network, you will develop a functional GPCR assay pipeline to support the identification of modulators and understand their mode of action. Promising pharmacological modulators can ultimately be tested in cellular and in vivo models of disease.

Planned secondments will provide hands‑on experience in in vitro assay validation and in vivo characterisation of lead molecules.

You will use molecular receptor pharmacology techniques to investigate the orphan GPCR and its potential as a therapeutic target. Your work will include:

• Developing cell‑based bioluminescent and fluorescent biosensor assays to detect GPCR signalling events in different cellular models.
• Investigating receptor activation and signalling mechanisms.
• Developing and applying high‑throughput screening assays to identify potential drug‑like modulators.
• Using FDA‑approved drug library repurposing and fragment‑based drug discovery to identify and characterise promising compounds.
• Contributing to the development of a functional GPCR assay pipeline and the evaluation of lead molecules in collaboration with POLARIS partners.

We are looking for a candidate with a Master's degree in pharmaceutical sciences, molecular neuroscience, biomedical sciences, medicinal chemistry or a comparable life sciences discipline. You bring:

• Relevant experience in molecular pharmacology and/or receptor biochemistry, including cell culture or cell‑based signal‑transduction assays using fluorescent or bioluminescent biosensors.
• A strong interest in GPCR biology and/or the development of cell‑based assays using high‑throughput microplate readers and robotics.
• Experience with drug discovery is an advantage.
• Experience with molecular biology techniques and fluorescence microscopy is an advantage.
• An accurate and reproducible approach to research, with strong analytical, communication and organisational skills and the ability to collaborate across different research environments.

Fluency in spoken and written English is essential.

Eligibility criteria Important, strict eligibility criteria according to MARIE SKLODOWSKA CURIE programs apply to applicants:

• You must not already hold a doctoral degree.
• At the time of the application deadline, you must have a Master's degree and must not have resided or carried out your main activity (work, studies, etc.) in the Netherlands for more than 12 months in the three years prior to the contract start date.
• You will participate in the network‑wide training programme of POLARIS, which includes (international) training activities and planned secondments with POLARIS partner organizations throughout the EU.

Additional Information

• A gross monthly base salary between €3,204 and €4,051, based on a full‑time 38‑hour working week, supplemented by an MSCA Doctoral Network allowance.
• 8% holiday allowance and an 8.3% year‑end bonus.
• 41 days of annual leave based on a full‑time employment contract.
• An initial one‑year employment contract, with the intention to extend this in accordance with the applicable conditions.
• A pension scheme through the ABP pension fund.
• A travel allowance of up to €0.23 per kilometre.
• Disability insurance.
• The opportunity to participate in the international POLARIS doctoral training network, including network‑wide training activities, secondments and transferable‑skills development.

The application deadline is 5 October 2026. We aim to select the final candidate in October/November 2026.

Applicants may, where appropriate and in line with the recruitment procedure, also be considered for other POLARIS doctoral candidate positions that better match their profile and interests. You can highlight up to two additional POLARIS projects of interest in your application letter. This does not replace your application to the primary position, but helps the consortium identify potential alternative matches where relevant.