Research Scientist/Senior Research Scientist

Biopatrika; Bengaluru; On-site; INR 900,000 - 1,200,000; Full time

Job summary

Biopatrika is seeking a Research Scientist/Senior Research Scientist in Bengaluru to design and optimize in vitro biochemical assays for screening small molecules, biologics, or proteins against therapeutic targets. The role covers assay development, validation, and screening across HTS/MTS platforms.

The candidate will characterize targets, tune reaction conditions, and ensure data integrity through rigorous analysis and SOP compliance.

Qualifications

• MSc or MTech in Biochemistry, Biotechnology, or Microbiology.
• Experience in designing and validating in vitro biochemical assays.
• Exposure to HTS/MTS screening workflows.

Responsibilities

• Design and optimize enzymatic, binding, and functional assays.
• Characterize target enzymes and binding parameters (Km, Vmax, KD, IC50).
• Optimize reaction conditions for robust, low-variability readouts.
• Conduct HTS/MTS campaigns in 96–1536-well formats.
• Operate and maintain plate readers and automation tools.
• Analyze screening data and generate assay quality metrics.
• Draft SOPs and support assay transfer across teams.

Skills

Assay design

HTS/MTS

Biochemical assays

Plate readers

Automation

Data analysis

Education

MSc/MTech

Tools

GraphPad Prism

Dotmatics

ClARIOstar

Job description

# Research Scientist/Senior Research ScientistJobBangalore, IndiaOctober 15, 2026Full Time### Job Overview* Date Posted October 15, 2026* Location Bangalore, India* Expiration date November 14, 2026* Click to apply: https://www.linkedin.com/posts/srinivas-reddy-madapa-a73902373\\_we-are-hiring-position-research-scientist-share-7516014267884666880-7ees/### Job DescriptionWe are Hiring!!! Position: Research Scientist/Senior Research Scientist – In Vitro Biochemical Assay Design & Optimization, Assay Development & Screening, The role is responsible for designing, optimizing, validating, and executing biochemical and biophysical assays to screen small molecules, biologics, or recombinant proteins against therapeutic drug targets (02 Vacancies) Department: In Vitro Biochemical Assay Design & Optimization, High-Throughput & Medium-Throughput Screening (HTS / MTS), High-Throughput Instrumentation & Automation Experience: 2 – 4 / 5-8 years Location: Bommasandra, Bengaluru, Karnataka, India Qualifications: M.Sc/M.Tech (Biochemistry/Biotechnology/Microbiology) Interested candidates can share their resume at [e-mail] (main ID), [e-mail], bachwellpharma@849ayushiLearnHindiCore Job Responsibilities1. In Vitro Biochemical Assay Design & Optimization Assay Development: Design and establish robust in vitro enzymatic, binding, and functional assays using various detection technologies (e.g., Absorbance, Fluorescence, TR-FRET, FP, Luminescence, AlphaLISA, and SPR). Target Characterization: Characterize target enzymes, kinases, proteases, or receptor proteins by determining kinetic parameters (K\\_m, V\\_{max}, k\\_{cat}) and binding affinity parameters (K\\_d, IC\\_{50}, EC\\_{50}). Parameter Tuning: Systematically optimize reaction conditions, including enzyme/substrate concentrations, incubation times, buffer composition, pH, salt strength, detergents, and DMSO tolerance to achieve robust signal-to-background ratios and low variability.2. High-Throughput & Medium-Throughput Screening (HTS / MTS) Compound Screening: Execute primary, secondary, and hit-validation screening campaigns (in 96-, 384-, or 1536-well microplate formats) for synthetic chemical libraries, natural products, or biologic drug candidates. Assay Robustness: Mechanism of Action (MoA) Studies:3. High-Throughput Instrumentation & Automation Plate Readers & Detection Systems: Operate, calibrate, and maintain multi-mode microplate readers (e.g., EnVision, PHERAstar, SpectraMax, CLARIOstar).Automated Liquid Handlers: Program and utilize automated liquid handling workstations (e.g., Tecan Freedom EVO/Fluent, Hamilton STAR, ECHO Acoustic Liquid Handler, Agilent Bravo) for serial dilutions and automated plate prep.Equipment Maintenance: 4. Data Analysis, Informatics & ReportingQuantitative Analysis: Process raw screening data using specialized curve-fitting and assay informatics software (e.g., GraphPad Prism, ActivityBase, Dotmatics, CDD Vault) to derive $IC\\_{50}$ / $EC\\_{50}$ curves and hill slopes. Data Integrity & Documentation: Cross-Functional Communication: 5. Laboratory Management & SOP Maintenance SOP Authoring: Draft, update, and review technical SOPs, validation protocols, and assay transfer guidelines. Reagent Procurement & Quality: Lab Safety & Compliance: