Research Associate: Protein Seeding & Neuropathology

Imperial College London; Greater London; On-site; GBP 42,000 - 55,000; Full time

Job summary

Imperial College London in London, UK, invites applications for a Research Associate in Neuropathology and Protein Seeding Assays to join an Alzheimer's Research UK-funded pilot project.

You'll establish RT-QuIC assays, develop isSID assays on human brain tissue, work with post-mortem tissue and CSF, perform immunohistochemistry, quantitative microscopy, and contribute to publications and grant applications.

Qualifications

• PhD (or near completion) in neuroscience, neuropathology, molecular biology, protein biochemistry or a related discipline.
• Experience working with protein-focused experimental research.
• Experience with immunohistochemistry, immunofluorescence or related tissue-based methodologies.
• Experience with quantitative microscopy and image analysis.
• Experience designing, troubleshooting and optimising laboratory assays.
• Ability to independently plan and execute experimental work.
• Evidence of scientific productivity through publications, preprints or equivalent outputs.

Responsibilities

• Establish and optimise RT-QuIC assays for TDP-43 and beta-amyloid using human brain tissue and CSF.
• Develop and apply novel isSID assays for beta-amyloid and TDP-43 on human post-mortem brain tissue sections.
• Prepare and characterise human brain homogenates and other biological samples from neurodegenerative disease cohorts.
• Perform immunohistochemistry, immunofluorescence and quantitative microscopy studies.
• Generate and analyse whole-slide imaging and confocal microscopy datasets.
• Correlate seeding activity with neuropathological features and associated clinical information.
• Optimise experimental workflows and establish standard operating procedures for newly developed assays.
• Prepare manuscripts for publication and present findings at scientific meetings.
• Contribute to future funding applications arising from this project.

Skills

Protein aggregation

Neuropathology

Quantitative imaging

Assay development

Experimental planning

Independent work

Scientific productivity

Education

PhD or near completion in neuroscience/ neuropathology/ molecular biology/ protein biochemistry

Tools

RT-QuIC

PMCA

Immunohistochemistry

Immunofluorescence

Quantitative image analysis

Job description

Imperial College London in London, UK, invites applications for a Research Associate in Neuropathology and Protein Seeding Assays to join an Alzheimer's Research UK-funded pilot project.

You'll establish RT-QuIC assays, develop isSID assays on human brain tissue, work with post-mortem tissue and CSF, perform immunohistochemistry, quantitative microscopy, and contribute to publications and grant applications.