Research Associate on the structural biology of bacterial secretion systems

Imperial Enterprise; Greater London; Vor Ort; GBP 45.000 - 49.000; Vollzeit

Zusammenfassung

Imperial College London seeks a structural biologist to lead a project on bacterial secretion systems, including Tad and Type 4 pilus systems. You will progress from cloning through purification to structure determination using cryo-EM and related methods.

Applicants should have a PhD in structural biology with a strong publication record; experience in membrane proteins and advanced microscopy is desirable. This is a full-time, fixed-term role funded by an 8-year Wellcome Trust Discovery award.

Qualifikationen

• PhD in structural biology or equivalent required.
• Experience with protein purification and structure determination workflows.
• Strong background in cryo-EM or X-ray crystallography or NMR.
• Experience with membrane protein complexes is desirable.

Aufgaben

• Lead your own project from initial cloning through to protein purification, structure determination and mechanistic dissection.
• Work on challenging multi-component membrane complexes in vitro and in cells.
• Apply structural biology techniques including cryo-electron microscopy, tomography and related methods.

Jobbeschreibung

Research Associate on the structural biology of bacterial secretionsystems

We are seeking to recruit an enthusiastic and motivated structural biologist to join the group of Prof Harry Low, Department of Infectious Disease ( www.thelowlab.org ).

The research project focuses on determining the molecular mechanism of bacterial secretion systems including the Tad and Type 4 pilus systems. The position is funded by an 8 year Wellcome Trust Discovery award.

What you would be doing

• You will be leading your own project working from initial clones through to protein purification, structure determination and biochemical/biophysical mechanistic dissection.
• You will be working on challenging multi-component membrane complexes both in-vitro and in-cell. Specific methodology will include a range of structural biology techniques including cryo-electron microscopy (SPA, in-situ SPA and tomography), and TIRF microscopy combined with single molecule FRET to probe protein dynamics.

What we are looking for

• You will have a PhD in structural biology, either cryo-EM, X-ray crystallography or NMR.

*Candidates who have not yet been officially awarded their PhD will be appointed as a Research Assistant within the salary range £45,399 - £48,876 per annum.

• Experience in the design and execution of workflow for structure determination of proteins, from molecular biology/biochemical experiments (e.g. DNA cloning and mutagenesis, protein purification) through to structural data collection, processing and model building is expected.
• Experience in cryo-EM workflows including sample preparation, data collection and processing is essential.
• Experience in light microscopy studies is desirable. You must have a strong research track record demonstrated by at least one first author publication in an international journal or bioRxiv.

What we can offer you

• An inclusive and friendly lab environment that aims to support you in your mission to make important discoveries and generate impactful publications.
• A well-funded lab underpinned by world class facilities.
• Opportunities to attend national and international conferences to showcase your work and grow your academic network.
• The opportunity to continue your career at a world-leading institution and be part of our mission to continue science for humanity.
• Sector-leading salary and remuneration package (including 39 days off a year and generous pension schemes).
• Be part of a diverse, inclusive and collaborative work culture with various staff networks and resources to support your personal and professional wellbeing .

Further information

This is a full-time (35 h/w) and fixed-term for 24 months, with the possibility to extend for a further 24 months.

Visa costs associated with moving to the UK will be covered.

If you require any further details about the role, please contact: Prof Harry Low – [e-mail]

Attached documents are available under links. Clicking a document link will initialize its download.

Please note that job descriptions are not exhaustive, and you may be asked to take on additional duties that align with the key responsibilities mentioned above.

We reserve the right to close the advert before the stated closing date, should we receive a high volume of applications. It is therefore advisable that you submit your application as early as possible to avoid disappointment.

If you encounter any technical issues while applying online, please don't hesitate to email us at [e-mail]. We're here to help.

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