PhD Studentship: Engineering Biology to Decode Gene Regulatory Networks in Lateral Root Initiation

University of East Anglia; Norwich; On-site; GBP 22,000 - 23,000; Full time

Benefits offered by this job

Stipend £21,805 per year

Research Training Support Grant £5,000

Fees paid (tuition)

Job summary

University of East Anglia is offering a PhD opportunity with the Guiziou Group at the Earlham Institute to apply engineering biology and single-cell genomic technology to understand lateral root initiation. You will build and optimise integrase-based genetic circuits in Arabidopsis and analyse them with fluorescence microscopy to reveal gene expression order in founder cells.

You will combine single-cell RNA sequencing with FACS to determine cell states in precise founder cell subpopulations,

Qualifications

• Undergraduate with a strong interest in engineering biology and plant science.
• Experience or coursework in molecular biology and microscopy valued.
• Interest in integrating DNA-based recording with single-cell approaches.

Responsibilities

• Build and optimise integrase-based genetic circuits in Arabidopsis.
• Analyse fluorescence to study gene expression timing and order.
• Use single-cell RNA sequencing (with FACS) to profile founder cell subpopulations.
• Develop critical thinking and scientific independence through the project.

Skills

Engineering biology

Plant biology

Single-cell RNA-seq

Arabidopsis genetics

Fluorescence microscopy

FACS

Genetic circuits

DNA-based recorders

Education

Undergraduate with strong background in engineering biology or plant science

Tools

Arabidopsis transformation

Single-cell sequencing

Protoplasting

Job description

Are you a motivated undergraduate with a passion for engineering biology and plant science?

We’re offering an exciting PhD opportunity with the Guiziou Group at the Earlham Institute using engineering biology and single-cell genomic technology to drive forward our understanding of lateral root initiation.

This PhD project aims to use DNA-based recorders (based on serine integrase) to reconstruct the gene expression programme during lateral root founder cell specification. You will combine this cutting-edge methodology with single-cell RNA sequencing to dissect spatiotemporal gene regulation of lateral root initiation.

You will build and optimise integrase-based genetic circuits in Arabidopsis to record the order in which genes of interest are expressed. Using fluorescence microscopy, you will analyse the resulting Arabidopsis lines to better understand the gene regulatory network underlying lateral root founder cell specification, with high spatiotemporal resolution.

You will also use single-cell RNA sequencing (combined with FACS) to determine the cell state of precise lateral root founder cell subpopulations marked with the engineered genetic circuit. During this project, you will develop your critical thinking and scientific independence, with the final part of the project being up to you to shape.

This project offers:

• Comprehensive training in engineering biology, automation, molecular biology, microscopy, plant transformation, protoplasting, FACS, single-cell RNA sequencing and analysis.
• Opportunities to present your work at international conferences and industry symposia.
• A vibrant, supportive research environment with excellent career progression: past students at the Earlham Institute became biotech CEOs, group leaders, and research scientists.
• A highly collaborative and supportive research community with access to training and career development opportunities across the Norwich Research Park.

You will become an expert in plant engineering biology through this project and take part in the mission of the Guiziou group, which is to have a tangible impact on sustainable agriculture.

This project is awarded with a 4-year Norwich Research Park Biosciences Doctoral Training Partnership PhD DTP studentship. The studentship includes payment of tuition fees (directly to the University), a stipend to cover living expenses (2026/27 stipend rate: £21,805, and a Research Training Support Grant of £5,000pa for each year of the studentship.